耐力运动对大鼠骨骼肌细胞葡萄糖运载体的影响  

Endurance exercise on glucose transporter in skeletal muscle of rats

作　　者：杨晓冰[1,2] 吴毅[1,2] 李益明[1,2] 李云霞[1,2] 占飞[1,2] 朱尚权[1,2] 张新堂[1,2] 平蓓芳[1,2] 

机构地区：[1]上海医科大学附属华山医院康复医学科 [2]中国科学院上海生化所

出　　处：《中华物理医学与康复杂志》1999年第1期17-19,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金

摘　　要：目的研究耐力运动对大鼠骨骼肌细胞葡萄糖运载体４（ＧＴ４）的影响。方法将ＳＤ大鼠随机分为二组：对照组和耐力运动组。耐力运动组大鼠进行６周游泳训练。制备ＧＴ４羧基端１２肽的多克隆抗体。以Ｗｅｓｔｅｒｎ印迹法对二组大鼠骨骼肌细胞ＧＴ４含量进行检测，同时检测大鼠血清胰岛素和血糖浓度。结果大鼠经过６周耐力运动，与对照组大鼠相比，骨骼肌细胞ＧＴ４含量增加３５．０％（Ｐ＜０．０１）。结论耐力运动可增加骨骼肌细胞内ＧＴ４含量。Objective To investigate the effect of endurance exercise on skeletal muscle cell glucose transporter 4 (GT4).Methods SD rats were randomly divided into two groups:endurance exercise group and control group.The rats in the former group were submitted to swim for 6 weeks.The polyclonal antibody against GT4 was obtained by immunizing rabbits and skeletal muscle GT4 was detected by Western blot analysis in both group rats.Results After six weeks of exercise training, skeletal muscle GT4 increased 35.0% in the experimental rats as compared with control ones( P < 0.01 ).Conclusion Exercise training can increase GT4 in the skeletal muscle,which may be one of the mechanisms for enhancing the glucose transport and uptake by skeletal muscle.

关 键 词：运动 耐力运动 骨骼肌细胞 葡萄糖 

分 类 号：G804.2[文化科学—运动人体科学]