儿童呼吸道感染者支原体、衣原体套式聚合酶链反应联合检测的研究  

Study on combined detection of M.genitalium, M.pneumoniae and C.pneumonia by nested polymerase chain reaction in respiratory tract infections in pediatric patients

作　　者：陆亚华[1] 孟平[1] 丁雁[1] 李璐[1] 

机构地区：[1]苏州医学院第三附属医院儿科

出　　处：《中华结核和呼吸杂志》1999年第4期214-216,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的探讨儿童呼吸道感染者的生殖支原体（Ｍｇ）、肺炎支原体（Ｍｐｎ）、肺炎衣原体（Ｃｐｎ）感染以及混合感染情况。方法采用套式聚合酶链反应（ＰＣＲ）检测技术，检测２３１例儿童咽试子标本的ＭｇＤＮＡ、ＭｐｎＤＮＡ、ＣｐｎＤＮＡ，并对Ｃｐｎ、Ｍｇ各２例阳性标本和其相对应的国际典型株作了ＤＮＡ测序分析。结果２３１例患儿的Ｍｇ、Ｍｐｎ、Ｃｐｎ感染阳性率分别是２２．５％、３０．７％、３２．９％，混合感染阳性率分别为：Ｍｇ＋Ｍｐｎ为５．２％，Ｍｇ＋Ｃｐｎ为２．６％，Ｍｐｎ＋Ｃｐｎ为１０．８％，Ｍｇ＋Ｍｐｎ＋Ｃｐｎ为８．７％。其中２例Ｃｐｎ阳性标本与国际典型株进行ＤＮＡ测序对比完全相同，１例ＭｇＤＮＡ有一个碱基突变，同源性达９９．８％，另１例完全相同。结论Ｍｇ可能是儿童呼吸道感染的病原体，病原体并可存在Ｍｇ、Ｍｐｎ。Objective To study the respiratory tract infections by Mg, M. genitalium (Mpn), Cpn, and co infections by these agents in pediatric patients. Methods Mg DNA, Mpn DNA and Cpn DNA were detected in respiratory tract specimens obtained by throat swab from 231 children by the nested polymerase chain reaction (PCR) technique. DNA sequencings were detected and analysed between every two positive specimens of Mg and Cpn and typical strains. Results Positive rates of Mg DNA、Mpn DNA and Cpn DNA by nested PCR in 231 children were 22.5% , 30.7% and 32.9%. The Positive rates of co infection were 5.2% (Mg and Mpn), 2 6% (Mg and Cpn), 10.8% (Mpn and Cpn) and 8.7% (Mg and Mpn and Cpn). There was only one base mutation in 1 Mg DNA. Other DNA sequencings were same as those from model strains. The homogeneity was 99.8%. Conclusions Mg could be one of the pathogens in respiratory tract infections in pediatric patients. There exists co infection by Mg, Mpn and Cpn.

关 键 词：儿童 呼吸道感染 支原体 衣原体 聚合酶链反应 

分 类 号：R725.604[医药卫生—儿科]