内源性一氧化碳在小儿哮喘发病机制中的作用初探  

Evidence of increased endogenous carbon monoxide production in asthma

作　　者：蒋东波[1] 史源[1] 李华强[1] 潘捷[1] 沈际皋[1] 姚忠凯[1] 刘萍[1] 覃世文[1] 潘凤[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所

出　　处：《中华结核和呼吸杂志》1999年第4期231-233,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：第三军医大学校级科研基金

摘　　要：目的探讨一氧化碳（ＣＯ）等在小儿哮喘发病机制中的作用及意义。方法应用Ｃｈａｌｍｅｒｓ血红蛋白结合及联二亚硫酸盐还原法检测了２２名正常儿童、２６例支气管哮喘患儿血浆ＣＯ水平，同时测定了一氧化氮（ＮＯ）和肿瘤坏死因子α（ＴＮＦα）水平，并在动物模型上进行验证。结果哮喘患儿急性发作期血浆ＣＯ、ＮＯ２／ＮＯ３和ＴＮＦα水平与对照组比较，ｔ值分别为８４２１，３０８８，１５４３２，（Ｐ均＜０．０１），缓解期ＣＯ和ＮＯ２／ＮＯ３水平恢复正常（Ｐ均＞０．０５），而ＴＮＦα水平与对照组比较差异有显著性（Ｐ＜０．０１）。哮喘患儿血浆ＣＯ与ＮＯ２／ＮＯ３水平呈显著正相关（ｒ＝０．４７６，Ｐ＜０．０５），而与ＴＮＦα相关不显著（ｒ＝０．２６８，Ｐ＞０．０５）。过敏性哮喘豚鼠血浆ＣＯ和ＴＮＦα水平与对照组比较，ｔ值分别为３６９４，７４５４，（Ｐ均＜０．０１），而ＮＯ２／ＮＯ３水平变化差异无显著性（Ｐ＞０．０５）。过敏性哮喘豚鼠支气管肺泡灌洗液（ＢＡＬＦ）中ＣＯ、ＮＯ２／ＮＯ３和ＴＮＦα水平的ｔ值分别为３０４９，５６０９，５９５５，（Ｐ＜０．０５～００１）。糖皮质激素组血浆及ＢＡＬＦ中?Objective To investigate the role of carbon monoxide (CO) in the pathogenesis of asthma. Methods We examined CO, nitrite and nitrate (NO 2/NO 3) and tumor necrosis factor alpha (TNF α) levels in plasma of 26 asthmatic children and 22 matched normal children. At the same time, we examined the plasma and bronchoalveolar lavage fluid (BALF) CO, NO 2/NO 3 and TNF α levels in guinea pigs with allergic asthma. Results Asthmatic children in acute attack stage had increased levels of CO, NO 2/NO 3 and TNF α in plasma compared with normal control subjects (all P <0.01), while in stable stage plasma CO and NO 2/NO 3 reduced to normal levels (both of the P >0.05), but TNF α was still higher than the normal controls ( P <0.01). Guinea pigs with allergic asthma had increased amounts of CO and TNF α in plasma and CO, NO 2/NO 3 and TNF α in BALF as compared with normal control subjects ( P <0.05～0.01), but no changes in levels of circulating NO 2/NO 3 ( P >0.05) were found. There was no difference between glucocorticoid treated asthmatic guinea pigs and normal controls in levels of CO, NO - 2/NO - 3, and TNF α. The elevation of plasma CO correlated with elevated NO 2/NO 3 level in asthmatic children ( P <0.05), so did in the BALF in allergic guinea pigs. Conclusions Our present data showed that endogenously produced CO was increased during the course of asthma, which suggested that the role of CO in asthma might worth further study.

关 键 词：一氧化碳 哮喘 儿童 发病机制 

分 类 号：R725.622.5[医药卫生—儿科]