c-myb对大鼠黄体细胞孕酮和雌二醇生成的影响  

Effect of c-myb on the Progesterone and EstradiolProduction of Rat Luteal Cells

作　　者：郑月慧[1,2] 方廉[1,2] 况海斌[1,2] 邹桂华[1,2] 

机构地区：[1]江西医学院生理学教研室 [2]江西医学院病理学教研室

出　　处：《基础医学与临床》1999年第2期65-68,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：本文观察了反义ｃ－ｍｙｂＯＤＮ对黄体细胞ｈＣＧ诱导的Ｐ和Ｅ。产生的影响，并探讨了与二了酰ｃＡＭＰ和ｖｅｒａｐａｍｉｌ的关系。结果发现，反义０．ｍｙｂＯＤＮ能呈剂量相关方式抑制黄体细胞ｈＣＧ诱导的Ｐ和民的产生，同时使ｃ．ｍｙｂ蛋白染色阳性的黄体细胞百分数下降：而无义ｔａｔＯＤＮ没有相应的作用。当１０４ｍｏｌｌＬ二丁酰ｃＡＭＰ与反义ｃ．ｍｙｂＯＤＮ共同作用时能明显逆转反义Ｃ．ｍｙｂＯＤＮ对产生和ｃ．ｍｙｂ表达的抑制作用。而１０‘ｍｏｌｌＬｖｅｒａｐｍｉｌ与反义０．ｍｙｂＯＤＮ共同作用时，对反义０．ｍｙｂＯＤＮ对产生和０．ｍｙｂ表达的抑制作用具有协同效应。表明，０．ｍｙｂ与黄体细胞ｈＣＧ诱导的Ｐ和产生密切相关；ｃＡＭＰ和Ｃａ２”能通过调节ｃ。The effects of c-myb antisense oligodeoxynucleotides (antisense c-myb ODN) on the hCG-induced progesterone and estradiol production of isolated rat luteal cells and their effect of cAMP andCa2+ were investigated. Antisense c-myb ODN could inhibit the hCG-induced progesterone andestradiol production in a dose-dependent manner, the percentages of luteal cells stained for c-mybprotein were also significantly decreased; while nonsense tat ODN had no similar effective.Futherexperiments showed that dbcAMP 10`-4mol/L could increase the percentage of luteal cells stained for c-myb protein, and increase the hCG-induced P and E, production. When dbcAMP was given incombination with antisense c-myb ODN, the inhibitory effect of antisense c-nlyb ODN upon hCG-induced P and E, production was reversed,the percentage of c-myb protein,positive luteal cells werealso increased.However, verapamil (l0-6 mol/L) had opposite effects on c-myb expression and hCG-induced P and E, production as compared with dbcAMP.The results implied that cAMP and Ca2+_might be involved in regulating the expression of c-myb protooncogene,in luteal cells and then regulateluteal cell function.

关 键 词：C-MYB 孕酮 雌二醇 黄体细胞 反义ODN 

分 类 号：R339.22[医药卫生—人体生理学]