MMP-2及TIMP-1、TIMP-2在自然流产绒毛和蜕膜组织中的表达及意义  

Expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1,2 in chorionic villi and deciduas from women with spontaneous abortion

作　　者：吕荟明[1] 孙状壮[1] 李力男[1] 宋巍[1] 王卓然[1] 

机构地区：[1]哈尔滨医科大学附属第一医院生殖医学科,黑龙江哈尔滨150001

出　　处：《中华疾病控制杂志》2010年第7期650-652,共3页Chinese Journal of Disease Control & Prevention

基　　金：黑龙江省自然科学基金(D2007-71)

摘　　要：目的探讨基质金属蛋白酶-2(MMP-2)及金属蛋白酶组织抑制剂-1(TIMP-1)和金属蛋白酶组织抑制剂-2(TIMP-2)在早孕自然流产绒毛和蜕膜组织中的表达及意义。方法采用S-P免疫组织化学染色的方法,对31例自然流产者(实验组)及36例人工流产者(对照组)绒毛及蜕膜组织中MMP-2和TIMP-2的表达进行分析;采用逆转录聚合酶链反应(RT-PCR)技术对绒毛组织中MMP-2及TIMP-1的mRNA表达量进行分析。结果无论是绒毛组织还是蜕膜组织中MMP-2的表达两组之间均无统计学差异;但实验组两种组织中的TIMP-2强阳性(Ⅲ级)表达率均显著低于对照组(P<0.01);实验组TIMP-1 mRNA的表达量显著低于对照组(P<0.05)。结论自然流产绒毛和蜕膜组织中MMP-2的正常表达及TIMP-1、TIMP-2的低表达导致MMP-2与TIMP-1及TIMP-2的比值升高,该比值失调可能与自然流产发生有关。Objective To investigate the expression of matrix metalloproteinase 2（MMP-2）,tissue inhibitor of metalloproteinase 1（TIMP-1） and tissue inhibitor of metalloproteinase 2（TIMP-2） in chorionic villi and deciduas from spontaneous abortion women.Methods Immunohistochemical method（S-P） was employed to detect the expression of MMP-2 and TIMP-2 in chorionic villi and deciduas from women with spontaneous abortion（31 cases） and normal pregnant women（36 cases）.Reverse transcriptase-ploymerase chain reaction（RT-PCR） was used to detect mRNA expression levels of MMP-2 and TIMP-1 in chorionic villi.Results There was no significant difference of MMP-2 expression in the two groups.While the strong positive（degree Ⅲ） expression rate of TIMP-2 in chorionic villi and deciduas from abortion group was significantly lower than that in the normal pregnant group（P0.01）,and the mRNA expression level of TIMP-1 was significantly lower than that in the control group too（P0.05）.Conclusions The higher ratio of MMP-2 to TIMP-1 or TIMP-2 in chorionic villi and deciduas,as a result of the normal expression of MMP-2 and the decreased expression of TIMP-1 and TIMP-2,may contribute to the spontaneous abortion.

关 键 词：基质金属蛋白酶2 金属蛋白酶组织抑制剂 流产 自然 

分 类 号：R181.26[医药卫生—流行病学] R714.21[医药卫生—公共卫生与预防医学]