异长春花碱脂质体中主药的含量以及包封率的测定  

Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes

作　　者：李鑫[1] 程岚[1] 李学涛[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中国实验方剂学杂志》2010年第11期50-52,55,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁中医药大学青年药学人才基金(yxrc0911)

摘　　要：目的:建立异长春花碱脂质体中主药含量以及脂质体包封率的测定方法。方法:采用高效液相色谱法测定药物含量,依利特C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.06 mol.L-1磷酸二氢钾(38∶62);流速1.0 mL.min-1;检测波长215 nm;柱温30℃;采用葡聚糖凝胶法分离异长春花碱脂质体游离药物以测定包封率。结果:异长春花碱在2.08～20.8μg.mL-1线性良好(r=0.999 9),平均加样回收率98.64%(RSD 1.09%),所测脂质体的平均包封率为86.83%。结论:该方法准确可靠,简单快速,重复性好,可用于异长春花碱脂质体药物含量及包封率的测定。Objective：To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes.Method：The HPLC analysis was carried out on a ELITE C18（4.6 mm × 250 mm,5 μm） column with mobile phase consisted of acetonitrile-0.06 mol.L-1 KDP buffer（38∶ 62） at a flow rate of 1.0 mL.min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination.Result：The 1inear range of vinorelbine was 2.08 ～ 20.8 μg（r = 0.999 9）.The average recovery was 98.64%（RSD 1.09%）.The average entrapment efficiency was 86.83%.Conclusion：This method is simple,precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.

关 键 词：异长春花碱 脂质体 含量测定 包封率 

分 类 号：R283.6[医药卫生—中药学]