HPLC测定清热消炎宁胶囊中异秦皮啶与迷迭香酸含量  

Determination Content of Isofraxidin and Rosmarinic Acid in Qingre Xiaoyanning Capsules by HPLC

作　　者：黎雄 杨柳 曾星 邓远辉 高智能[2] 

机构地区：[1]广州中医药大学第二附属医院,广州510200 [2]广东药学院,广州510006

出　　处：《中国实验方剂学杂志》2010年第11期53-55,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定消炎宁胶囊中异秦皮啶与迷迭香酸含量的高效液相色谱分析方法。方法:色谱柱WatersXBridge C18柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%磷酸(21∶79),等度洗脱;检测波长340 nm;流速1 mL.min-1。结果:异秦皮啶在0.32～3.2μg,迷迭香酸在0.256 6～2.566μg与峰面积呈良好的线性关系(r=0.999 8,0.999 9);平均加样回收率分别为97.3%,98.9%,RSD分别为1.6%,1.3%。结论:该法简便、重复性好、各成分分离良好,建立的方法可更有效地控制清热消炎宁胶囊的质量。Objective：To establish an HPLC method for simultaneous determination of isofraxidin and rosmarinic acid in Qingre Xiaoyanning Capsules.Method：A HPLC method was used with a Waters XBridge C18 column（4.60 mm × 250 mm,5 μm） and acetonitrile-0.1% phosphoric acid（21∶ 79） as the mobile phase.The flow rate was 1.0 mL.min-1 and the detection wavelength was 340 nm.Result：The linear ranges of isofraxidin and rosmarinic acid were 0.32-3.2 μg（r = 0.999 8） and 0.256 6-2.566 μg（r = 0.999 9）,respectively.The average recoveries（n = 6） were 97.3%（RSD 1.6%） and 98.9%（RSD 1.3%）,respectively.Conclusion：This HPLC method is simple,accurate and relilable,and can be used for the quality control of Qingre Xiaoyanning Capsules.

关 键 词：清热消炎宁胶囊 异秦皮啶 迷迭香酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]