RP-HPLC测定苗药黑白保肝清颗粒中槲皮素的含量  

Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC

作　　者：杨立昌[1] 乙引[1] 林俊清[1] 张习敏[1] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001

出　　处：《中国实验方剂学杂志》2010年第11期64-66,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化科技产业研究开发专项(黔科合社字[2009]5043号);贵州省科技创新人才团队建设项目(黔科合人才团队[2009]4007号)

摘　　要：目的:建立苗药黑白保肝清颗粒中槲皮素总量的测定方法。方法:采用RP-HPLC法,Diamonsil(TM)钻石C18柱(4.6 mm×250 mm,5μm),以甲醇-0.2%磷酸(56∶44)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长370 nm。结果:在0.020 6～0.133μg进样量范围内,槲皮素含量与峰面积呈很好的线性关系(r=0.997),平均回收率为98.2%,RSD为1.70%。5批样品中槲皮素总量均值为1.44 mg.g-1。结论:该方法简便,准确,重复性好,可用于苗药黑白保肝清颗粒中槲皮素总含量测定。Objective：To establish a method to determine quercetin in Heibai Baoganqing Granules of Mao ethnomedicine by HPLC.Method：HPLC was used.The column was Diamonsil（TM） C18（4.6 mm × 250 mm,5 μm）.The mobile phase consisted of methanol-0.2% phosphoric acid（56∶ 44）,with the flow rate of 1.0 mL.min-1and the wavelength was at 370 nm.Result：Quercetin was linear in the range of 0.020 6 ～ 0.133 μg.The average recovery was 98.2%.The average content of quercetin in five samples was 1.44 mg.g-1.Conclusion：This method is accurate,simple and reproducible,and can be used for the determination of quercetin in Heibai Baoganqing Granules.

关 键 词：黑白保肝清颗粒 槲皮素 含量测定 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]