大鼠在体单向肠灌流法进行丹参素、丹酚酸B的肠吸收研究  

Intestine Absorption Study on Danshensu and Salvianolic Acid B with Rat in situ Single Pass Perfusion Model

作　　者：张英丰[1] 李玉洁[2] 杨庆[2] 翁小刚[2] 董宇[3] 朱晓新[2] 

机构地区：[1]广州中医药大学,广州510006 [2]中国中医科学院中药研究所,北京100700 [3]中国中医科学院广安门医院,北京100053

出　　处：《中国实验方剂学杂志》2010年第11期96-100,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重点项目(30930114);国家自然科学基金项目(30701097);国家“十一五”科技支撑计划课题(2006BAI08B04-4);中国博士后基金项目(20070420509)

摘　　要：目的:考察丹参水溶性成分-丹参素和丹酚酸B的肠吸收特性。方法:采用大鼠在体单向肠灌流法,测定不同质量浓度丹参素和丹酚酸B在不同肠段、不同浓度的吸收分数(Fa,%)和表观渗透系数(Papp)。结果:丹参素和丹酚酸B在各肠段的吸收均比较差,十二指肠和空肠吸收较回肠和结肠略好,质量浓度、肠段对Fa和Papp无明显影响。结论:丹参素和丹酚酸B在全肠道以被动扩散方式吸收,且吸收比较差,无特定吸收部位,不受药物浓度的影响。Objective：To study the absorption of salvianolic acid B and danshensu.Method：In situ single pass perfusion model was used and the concentration of salvianolic acid B and danshensu in perfusate were determined by HPLC in combination with ultraviolet detector.Result：The apparent penetration coefficient（Papp） and absorption percentage（Fa） of danshensu and salvianolic acid B were all low at different concentration in four different regions of intestine of rat（duodenum,jejunum,ileum,colon）.The absorption rate of dashensu was faster than salvianolic acid B in this study.The values of the Fa a and the Papp were not significantly affected by concentration of perfusion solution and intestinal segments.Conclusion：The intestine absorption characteristics of salvianolic acid B and danhensu was poor at all segments of intestine in rats where there is no special ab sorption site and showed the passive diffusion process.

关 键 词：丹参素 丹酚酸B 肠吸收 在体单向肠灌流 

分 类 号：R285.5[医药卫生—中药学]