中药复方茯苓汤有效成分的初步研究  

A Preliminary Study on Effective Components of Traditional Chinese Medicine Fufang Fuling Tang

作　　者：王傲雪[1] 葛广波[2] 齐晓怡[1] 胡莹[2] 林玲[1] 林茂[1] 涂彩霞[1] 杨凌[2] 

机构地区：[1]大连医科大学附属二院,辽宁大连116027 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《中国实验方剂学杂志》2010年第11期120-124,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：大连市科委基金项目(2007E21SF182);科技部创新方法工作专项(2008IM020900)

摘　　要：目的:通过化学分析及不同中药组合的药效学实验揭示临床用于治疗湿疹的中药复方茯苓汤的主要有效成分及活性物质群。方法:采用超高速液相色谱-二极管阵列检测器-电喷雾-质谱(UFLC-DAD-ESI-MS)法分析复方茯苓汤的主要化学成分;在化学轮廓测定的基础上,依据主要物质峰的来源对中药组方进行了拆分并设立了4组中药组合(Ⅰ-Ⅳ),Ⅰ组为茯苓,泽泻,黄柏,栀子,赤芍,浮萍,当归,甘草8味中药组方后的水提物,Ⅱ组为栀子,赤芍,甘草3味中药组方的水提物,Ⅲ组为茯苓,当归2味中药组方的水提物,Ⅳ组为茯苓,栀子,赤芍,当归,甘草5味中药组方的水提物。用2,4-二硝基氟苯(DNFB)诱发的小鼠变应性接触性皮炎(ACD)为动物模型,比较ig不同中药组合(Ⅰ-Ⅳ)及阳性对照氢化可的松和阴性对照生理盐水的药效学指标,观察中药对小鼠耳质量、耳厚度、耳真皮炎性细胞浸润以及血清细胞因子的影响。结果:UFLC-DAD-ESI-MS代谢轮廓分析获得了复方茯苓汤中的11个主要化学成分信息,其中京尼平龙胆双糖苷、京尼平苷、芍药苷和甘草苷为其主要化学成分。与生理盐水组比较,各中药组和氢化可的松组对诱发皮炎前后小鼠的耳质量差、耳厚度差及真皮炎症细胞浸润的影响均有显著差异(P<0.05),其中,Ⅰ组、Ⅳ组和氢化可的松组更优(P<0.01)。结论:复方茯苓汤的主要药效成分多来源于栀子,赤芍,甘草,茯苓和当归5味中药。Objective：To analyze the chemical constitutes and effective components of Fufang Fuling Tang（FFFLT）,a traditional Chinese medicine shown to be effective in treating eczema.Method：A rapid method incorporating of ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra（UFLC-DAD-ESI-MS） profiling was developed for screening the chemical constitutes in FFFLT.Based on the different peaks from FFFLT samples,four herb groups（Ⅰ-Ⅳ） were established.Herb group I was composed of Poria cocos,Paeonia lactiflora Pall,Gardenia jasminoides,Glycyrrhiza uralensis,Alisma orientalis,Phellodendron amurense,Angelica sinensis and Spirodela polyrrhiza;groupⅡwas composed of P.lactiflora Pall,G.jasminoides,G.uralensis;group Ⅲ was composed of P.cocos and A.sinensis;and group Ⅳ was composed of P.cocos,P.lactiflora Pall,G.jasminoides,G.uralensis and A.sinensis.Sixty mice with allergic contact dermatitis（ACD） induced by DNFB were randomly divided into 6 groups,including Ⅰ-Ⅳ herb treatment groups（HTG）,positive control group（hydrocortisone treatment group,HCTG） and negative control group（physiological saline treatment group,PSTG）.The thickness and weight of mouse ears,the pathological changes in ear tissue and the serum levels of interferon（IFN）-γ,interleukin（IL）-4 and IL-10 were observed.Result：11 chemicals were analyzed and the most abundant constitutes from FFFLT were genipingentiobioside,geniposide,paeoniflorin and liquiritin.Compared with PSTG,significant decrease in ear swelling and dermal inflammatory infiltration were seen inⅠ-Ⅳ HTG and the HCTG（P 0.05）.ⅠHTG,ⅣHTG and HCTG showed better effects（P 0.01）.Conclusion：The pharmacodynamics of FFFLT in treating eczema mostly come from Paeonia lactiflora Pall,Gardenia jasminoides,Glycyrrhiza uralensis,Poria cocos and Angelica sinensis.

关 键 词：复方茯苓汤 皮炎 超高速液相色谱-二极管阵列检测器-电喷雾-质谱 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]