清营解表合剂防治流感免疫调节机制的研究  

Immune Protective Mechanism of Qingying Jiebiao Heji on Influenza

作　　者：孙喜稳[1] 吕翠霞[2] 张淼[1] 孙喜涛[3] 余斌[1] 张歌[1] 

机构地区：[1]暨南大学医学院,广州510632 [2]山东中医药大学,济南250355 [3]喀什师范学院,新疆喀什844007

出　　处：《中国实验方剂学杂志》2010年第11期161-164,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨清营解表合剂对流感病毒A/PR/8/H1N1感染小鼠免疫保护的作用机制。方法:以亚洲甲型鼠肺适应株A/PR/8/H1N1流感病毒小鼠滴鼻,建立感染模型。分别给予病毒唑、清营解表合剂,计算死亡保护率、延长生命率;测定小鼠胸腺指数和脾脏指数;流式细胞术检测小鼠外周血特异性细胞因子Th1(γ-干扰素,IFN-γ)/Th2(白介素-10,IL-10)表达水平。结果:清营解表合剂组小鼠死亡保护率和生命延长率分别为52.94%和37.64%,小鼠脾脏指数和胸腺指数明显提高。感染后小鼠外周血细胞的Th1型反应弱势表达,Th2细胞因子优势表达,Th1(IFN-γ)/Th2(IL-10)比值明显降低,Th1/Th2表达向Th2偏移。清营解表合剂组Th1细胞因子水平显著升高,且向空白组正常水平方向漂移。结论:清营解表合剂对感染小鼠具有明显的死亡保护作用,能够逆转流感病毒感染小鼠外周血因子Th1向Th2漂移的作用,通过免疫调节作用防治流行性感冒。Objective：To study the immune protective mechanism of Qingying Jiebiao Heji on A/PR/8 / H1N1 virus infected mice.Method：The influenza model in mice was established through dropping A /PR /8 /H1N1 virus into the nose of Kunming mice.Ribavirin and Qingying Jiebiao Heji were given to the infected mice respective-ly,then death protection and life prolongation were determined;thymus index and spleen index were observed;Th1（IFN-γ） /Th2（IL-10） expression in peripheral blood was measured by flow cytometry.Result：After being infec-ted,the death protection rate and life prolongation rate of Qingying Jiebiao Heji were 52.94% and 37.64% accord-ingly,the thymus index and spleen index were improved apparently.Th1cytokine IFN-γ attenuated and Th2cytokine IL-10 dominantly expressed.The expression of Th1（IFN-γ） /Th2（IL-10） was remarkably depressed and adjusted,Th2 expression of Qingying Jiebiao Heji group was markedly strengthened and Th1 / Th2 drifted towards the normal level.Conclusion：Qingying Jiebiao Heji has the effect of death protection on the A /PR /8 /H1N1 virus infected mice,it also can reverse its peripheral blood cytokine Th1drifting to Th2,and prevent influenza through immune reg-ulation.

关 键 词：流行性感冒 清营解表合剂 TH细胞 Γ-干扰素 白介素-10 

分 类 号：R285.5[医药卫生—中药学]