芭蕉根HPLC指纹图谱鉴别研究  

Identification of Musa basjoo by HPLC Fingerprint

作　　者：王祥培[1] 孙宜春[1] 靳凤云[1] 许士娜[1] 吴红梅[1] 黄婕[1] 

机构地区：[1]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2010年第12期30-33,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860387);贵州省科学技术基金(J20072138)

摘　　要：目的:建立芭蕉根的HPLC指纹图谱鉴别方法。方法:采用RP-HPLC-DAD法,以Diamonsil-C18(4.6 mm×250mm,5μm)色谱柱,流动相乙腈-0.05%磷酸水,采用梯度洗脱,流速1.0 mL.min-1,310 nm波长下测定芭蕉根、香蕉根及美人蕉根的指纹图谱,并作相似度比较分析。结果:建立了芭蕉根药材HPLC-UV指纹图谱,20个共有峰被标定,并对芭蕉根、香蕉根及美人蕉根药材的HPLC指纹图谱进行相似度比较,结果差异明显。结论:HPLC指纹图谱具有重复性好,特征性强,方法简便等特点,可用于芭蕉根药材的鉴别。Objective： To establish a HPLC fingerprint for the identification of Musa basjoo.Method： The analysis was carried out on a Diamonsil C18（4.6 mm × 250 mm,5 μm）with a mobile phase consisting of acetonitrile0.05% phosphoric acid,gradient eluent,the flow rate of 1.0 mL.min-1.The wave length was 310 nm,data were analyzed by fingerprint similarity evalution software to compare the similarity of samples.Result： The mutual mode to HPLC-UV fingerprints was estabilished,and the 20 mutual peaks were indicated.The similarities among the of Musa basjoo,Musa nana and Canna indica collected from different sources were obtained.There were apparent difference in fingerprint.Conclusion： HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Musa basjoo.

关 键 词：芭蕉根 鉴别 HPLC指纹图谱 

分 类 号：R284.1[医药卫生—中药学]