HPLC测定广西莪术不同炮制品超微细粉中牻牛儿酮的含量  

Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC

作　　者：韦相忠[1] 岳丽[1] 秦松梅[1] 劳深[1] 冯军[1] 陆秋凡[1] 李耀华[1] 

机构地区：[1]广西中医学院,南宁530001

出　　处：《中国实验方剂学杂志》2010年第12期45-47,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学基金项目莪术炮制工艺的热力学研究(桂科自0848008)

摘　　要：目的:研究广西莪术4种不同炮制品超微粉碎前后对其中牻牛儿酮含量的影响。方法:超微细粉的制备采用低温(-30±2)℃球磨技术,磨细至1.0～2.0μm尺寸;采用HPLC,色谱柱大连依利特Hypersil ODS2 C18柱(4.6 mm×250 mm,5μm);流动相乙腈-水(65∶35);紫外检测波长为210 nm。结果:不同炮制品超微细粉中牻牛儿酮的含量在粒径为75～53μm的普通粉末较高,而粒径为1.0～2.0μm的莪术超细粉末含量明显降低。结论:超微细粉的粒径对广西莪术炮制品中牻牛儿酮含量有明显影响,含量并不随粒度的减小而增加,为广西莪术不同炮制品提取工艺提供试验参考数据。Objective： To determine the content of germacrone from different processed products of Curcuma kwangsiensis before and after the superfine comminution.Method： With the ball milling technology at low temperatures（-30 ± 2） ℃,the size of the superfine powder was between 1.0-2.0 μm.The HPLC analysis was performed on Hypersil ODS2 C18（4.6 mm × 250 mm,5 μm）,the mobile phase was acetonitrile-water（65∶ 35）,the detection wavelength was at 210 nm.Result： The germacrone content of commom powder（75-53 μm） was higher than the content of the superfine powder（1.0-2.0 μm）.Conclusion： The size of superfine powder influenced the content of germacrone of the four different processed products of C.kwangsiensis evidently,and this offered referenced data for the different processed products of C.kwangsiensis.

关 键 词：广西莪术 超微细粉 牻牛儿酮 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]