从脑肠互动的角度研究痛泻要方治疗肠易激综合征的作用机制  

Mechanisms for Tongxie Yaofang to Treat Irritable Bowel Syndrome from Point of View of Interaction between Brain and Intestine

作　　者：李冬华[1] 白霞[1] 谢小磊[1] 耿建国[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中国实验方剂学杂志》2010年第12期118-121,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药科学技术研究专项课题(06-07ZQ01)

摘　　要：目的:从脑肠互动的角度研究痛泻要方治疗肠易激综合征(irritable bowel syndrome,IBS)的作用机制。方法:50只Wistar大鼠随机分为正常组、模型组、痛泻要方高剂量组、痛泻要方低剂量组、得舒特组。除正常组外,其余各组大鼠采用束缚制动加ig番泻叶的方法建立IBS大鼠模型,免疫组织化学法检测各组大鼠不同脑区核团c-fos蛋白表达,同时通过观察大鼠粪点数、玻璃小球排出时间及小肠墨汁推进率来检测大鼠结肠转运功能、小肠运动功能。结果:痛泻要方高剂量组与模型组相比,大鼠粪点数明显减少(P<0.05),玻璃小球排出时间减慢,小肠墨汁推进率明显降低(P<0.01),不同脑区核团c-fos阳性神经元灰度值明显增高(P<0.05),c-fos蛋白表达显著降低。结论:痛泻要方对IBS大鼠的脑肠轴功能紊乱有调控作用。Objective： To study the mechanisms of Tongxie Yaofang to treat irritable bowel syndrome from point of view of interaction between brain and intestine.Method： 50 Wister rats were divided randomly into normal group,model group,the large dose of Tongxie Yaofang group,the lower dose of Tongxie Yaofang group and the dicetel group.The rat model of irritable bowel syndrome was made by moving-restriction combined with taking senna decoction,The protein expressions of c-fos were detected by immunohistochemistry in different brain areas and nucleus in brain,the functions of colonic and small intestine were also evaluated by observing the counts of feces,the discharging times of the glass beads and the ink movement in the small intestine in rats.Result： The counts of feces in rats were decreased（P 0.05）,the discharging times of the glass beads were reduced and the ink movement in the small intestine were markedly decreased（P 0.01）,the gray values of c-fos protein in different brain areas and nucleus were significantly higher（P 0.05） in large dose Tongxie Yaofang group in comparison with model group,the protein expressions of c-fos in different brain areas in Tongxie Yaofang group were markedly decreased.Conclusions：Tongxie Yaofang has the effect of regulating the functions of the axis of brain and intestine.

关 键 词：肠易激综合征 痛泻要方 脑肠互动 C-FOS蛋白 

分 类 号：R285.5[医药卫生—中药学]