通络生骨胶囊促进兔膝关节全层软骨缺损修复的研究  

Tonglou Shenggu Capsule Restores Full-Thickness Articular Cartilage Defect of Rabbit's Knee: An Experimental Study

作　　者：唐芳根[1] 袁芬连[1] 钟敬亮[1] 刘家开[1] 

机构地区：[1]井冈山大学医学院,江西吉安343000

出　　处：《中国实验方剂学杂志》2010年第12期153-156,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察通络生骨胶囊对兔膝关节全层软骨缺损修复的作用。方法:建立兔膝关节髌股关节面全层关节软骨缺损模型,将其分为通络生骨胶囊低、中、高剂量(2,4,8 g·kg-1)治疗组,阳性药物(复方丹参片0.36 g·kg-1)对照组和模型组。造模成功后于第12周取材进行HE染色、甲苯胺蓝染色、苦味酸天狼猩红染色及扫描电镜检查。结果:模型组关节软骨缺损不能修复,复方丹参片组修复组织以纤维软骨为主,通络生骨胶囊各组修复组织为类透明软骨,以高剂量组修复质量最好,但与正常透明软骨仍有差距。结论:通络生骨胶囊能促进骨髓间充质干细胞对全层关节软骨缺损进行修复。Objective： To investigate the effect of Tonglou Shenggu Capsule for restoring the full-thickness articular cartilage defect in rabbit.Method： The model was established by drilling an articular cartilage defect of 5 mm in depth and 4 mm in diameter in the trochlea groove of the right femoral of New Zealand rabbits.Fifty rabbits were divided into five groups： model group,positive treatment group（Compound Danshen Tablet group） and three dosage groups of Tonglou Shenggu Capsule（low dose,middle dose and high dose）.Postoperativelly,the positive treatment group was lavaged with Compound Danshen Tablet,0.36 g.kg-1.d-1;the low dose group,middle dose group and high dose group were lavaged with Tonglou Shenggu Capsule of 2,4 and 8 g.kg-1 respectively;the model group was not given any medicine.All rabbits were sacrificed at twelve weeks after operation.The distal femoral portion was studied histologically using hematoxylin and eosin,toluidine blue,picric acid-Sirius red staining,and ultra-structure was investigated by scanning electron microscopy.Repairing tissues were analyzed using Wakitani＇ grading scale.Result： The full-thickness defects in rabbits could not restore by itself.The Compound Danshen Tablet could help the defects to restore,there were fibrocartilage cells and hyaline cartilage cells in the repairing tissues.Type I and type II collagens were found in the repairing tissues.Tonglou Shenggu Capsule could prompt the restoration of the defects.The major components are hyaline cartilage cells and type Ⅱ collagen.The quality of restoring was related with dosage.The restoring in high dose group was the best,but still different from the normal hyaline cartilage.Conclusion： Tonglou Shenggu Capsule could promote bone marrow mesenchymal cell to restore articular cartilage defect.

关 键 词：软骨缺损 骨髓间充质干细胞 通络生骨胶囊 

分 类 号：R285.5[医药卫生—中药学]