过氧化氢对小鼠睾丸生精细胞环氧化酶-2和Caspase-3表达的影响  

Effect of Hydrogen Peroxide on Expression of Cyclooxygenase 2 and Caspase-3 in Testicular Spermatogenic Cell of Mouse

作　　者：毛泽善[1] 杨杰[2] 袁向山[2] 杨志军[3] 文小军[4] 马全祥[2] 

机构地区：[1]新乡医学院形态学实验室,河南新乡453003 [2]新乡医学院组胚教研室,河南新乡453003 [3]新乡医学院化学教研室,河南新乡453003 [4]新乡医学院人体解剖学教研室,河南新乡453003

出　　处：《实用儿科临床杂志》2010年第18期1432-1433,1443,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省教育厅资助课题(2007310009);新乡市科委资助课题(08S061)

摘　　要：目的探讨过氧化氢(H2O2)摄入后小鼠睾丸生精细胞环氧化酶-2(COX-2)和Caspase-3表达的影响。方法健康雄性昆明种小鼠30只,随机分为对照组10只和实验组20只。对照组小鼠喂食自来水,实验组小鼠以0.3 g.L-1H2O2(0.3 g.L-1剂量组)和3.0 g.L-1H2O2(3.0 g.L-1剂量组)溶液喂食。30 d时处死各组小鼠,取出其睾丸,免疫组织化学链霉亲和素-生物素酶复合物染色法检测其生精细胞COX-2和Caspase-3表达的变化。结果与对照组比较,0.3 g.L-1剂量组和3.0 g.L-1剂量组COX-2、Caspase-3表达均显著升高,差异有统计学意义(Pa<0.05,0.01),与0.3 g.L-1剂量组比较,3.0 g.L-1剂量组COX-2无显著升高,Caspase-3显著升高(P<0.01)。结论 HO经口摄入可造成小鼠睾丸生精细胞的损伤。Objective To explore the effect of hydrogen peroxide（H2O2） on the expression of cyclooxygenase 2（COX-2） and Caspase-3 in mouse testicular spermatogenic cell.Methods Thirty healthy male mice of Kunming Genus were divided into 2 groups randomy： control group（n=10）,experimental group（0.3 g·L^-1 H2O2 group,n=10;and 3.0 g·L^-1H2O2 groups,n=10）.Control group ingested tap water,and mice in experimental groups ingested 0.3 g·L^-1or 3.0 g·L^-1H2O2 solution.On the 30th day,testis were quickly taken out,fixed in the Zamboni fixation solution,conventionally produced frozen section and stained,the expression of COX-2 and Caspase-3 were detected.Results Compared with the control group,the expression of COX-2 and Caspase-3 in 0.3 g·L^-1group and 3.0 g·L^-1group were significantly increased（Pa〈0.05,0.01）.The expression of COX-2 in 3 g·L^-1group was samilar to that in 0.3 g·L^-1group,and the expression of Caspase-3 in 3.0 g·L^-1H2O2 group was significantly increased compared with that in 0.3 g·L^-1group（P〈0.01）.Conclusion Harmful of mouse testicular spermatogenic cell can be caused by feeding H2O2.

关 键 词：过氧化氢 环氧化酶-2 CASPASE-3 生精细胞 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]