KATP通道开放剂Pinacidil诱导的超极化停搏对离体幼兔心肌的保护作用  

Myocardial protective effects of hyperpolarized cardioplegia induced with Pinacidil on isolated immature rabbits

作　　者：邓宏平[1] 程邦昌[1] 陈蕾[1] 王志维[1] 毛志福[1] 胡小平[1] 

机构地区：[1]武汉大学人民医院心血管外科,430060

出　　处：《中华实验外科杂志》2010年第9期1233-1235,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察Pinacidil诱导的超极化停搏对离体幼兔心肌保护的效果.方法 建立幼兔离体心脏灌注模型,分别以10℃标准St.Thomas液（Ⅰ组）、含Pinacidil 50 μmol/L的10℃ St.Thomas液（K＋5 mmol/L、Ⅱ组）和含Pinacidil 50 μmol/L的37 ℃ St.Thomas液（K＋5 mmol/L、Ⅲ组）诱导停跳,观察丙二醛（MDA）、血清心肌酶活性指标、超微结构及心功能变化.结果 再灌注20 min后MDA（nmol/mg蛋白）分别为97.88±37.09、70.19±12.73、143.36±77.48,Ⅱ组与Ⅰ、Ⅲ组间差异有统计学意义（P＜0.05及P＜0.01）,Ⅰ、Ⅲ组差异无统计学意义（P＞0.05）;肌钙蛋白Ⅰ（cTnI,μg/L）0.951±0.026、0.740±0.028、1.510±0.042,左心室最大压力变化速率（＋dp/dtmax,mm Hg/s）763.0±57.5、927.0±65.6、551.4±70.3,各组间差异有统计学意义（P＜0.01）;冠状动脉流量（CF,ml/min）3.9 ±0.3、6.0±0.8、3.1±0.6,Ⅱ组与Ⅰ、Ⅲ组间差异有统计学意义（P＜0.01）,Ⅰ、Ⅲ组差异有统计学意义（P＜0.05）.结论 超极化停搏对离体幼兔心肌保护效果优于传统去极化停搏,但仍须合并低温.Objective To observe the protective effects of hyperpolarized cardioplegia induced with Pinacidil on immature rabbit hearts. Methods Twenty-four immature rabbits of 3-4 weeks, weighing 350-450 g were randomly divided into three groups： hyperkalemic control group （ group Ⅰ ）, hyperpolurized group （ group Ⅱ）, and normothermic hyperpolarized group （ group Ⅲ ）. The cardioplegia was induced by St. Thomas solution with either traditional high potassium （ 16 mmol/L KCl, 10℃, group Ⅰ）, or pinacidil 50μmol/L （ 10℃ in groupⅡ and 37℃ in group Ⅲ） with 5 mmol/L KCl. The hearts were subjected to 60 min globally ischemic and followed by 20 rain reperfusion with 37 ℃ K-H. Serum aspartate transaminase （AST） , lactate dehydrogenase （LDH）, creatine kinase （CK） and cTnI, myocardial malondialdehyde （MDA）, and ultrastructural changes were examined. Preischemia and postreperfusion myocardial function was assessed by the percentage recovery of left ventricle developed pressure （LVDP）, left ventricle end-diastolic pressure （ LVEDP）, ±dp/dtmax, and coronary flow （CF）. Results After 20 rain reperfusion, MDA （nmoL/mg protein） in groupsⅠ ,Ⅱ, and Ⅲ was 97. 88±37. 09, 70. 19 ±12. 73 and 143. 36±77.48 respectively. There was significant difference between group Ⅱ and groups Ⅰ and Ⅲ （ P 〈 0. 05 and P 〈 0. 01 ）, but there was no significant difference between group Ⅰ and group Ⅲ （ P 〉0.05）. In groups Ⅰ , Ⅱ, and Ⅲ, cTnI （μg/L） was 0.951±0.026, 0.740 ±0.028 and 1.510 ±0. 042, ± dp/dtmax （ mm Hg/s） was 763.0 ±57.5,927.0 ± 65.6 and 551. 4 ± 70.3, respectively. There was significant difference among these groups （P 〈 0. O1 ） ; CF （ml/min） was 3. 9 ± 0. 3, 6. 0 ± 0. 8 and 3. 1 ±0. 6, respectively. There was significant difference between group Ⅱ and groups Ⅰ, Ⅲ （P 〈0. 01 ）, and between group I and group Ⅲ（P 〈0. 05）. Ultrastructural changes showed to serious damages in group Ⅲ, but there we

关 键 词：超极化停搏 未成熟心肌 心肌保护 

分 类 号：R654.2[医药卫生—外科学]