新福林增强人心房肌细胞肿胀激活的氯离子电流  

Phenylephrine enhanced swelling-activated chloride current in human atrial myocytes

作　　者：李业涛[1] 杜心灵[2] 

机构地区：[1]贵州省人民医院心外科,贵阳550002 [2]华中科技大学同济医学院附属协和医院心外科

出　　处：《中华实验外科杂志》2010年第9期1236-1238,共3页Chinese Journal of Experimental Surgery

基　　金：国家教育部留学回国人员基金资助项目（教外司留[2004]527）

摘　　要：目的 通过全细胞膜片钳技术研究α1-肾上腺素受体激动剂新福林对人心房肌细胞肿胀激活的氯离子电流（ICl.swell）的调节作用.方法 术中取冠脉搭桥患者右心耳组织,酶解分离出心房肌细胞.全细胞膜片钳技术记录氯离子电流.等渗、低渗台氏液灌流;分别加入新福林、氯离子电流阻滞剂4,4＇-二异硫氰基芪-2,2＇-二磺酸（DIDS）、α1受体阻断剂哌唑嗪,观察电流变化.结果 低渗台氏液灌流心房肌细胞,可记录到ICl.swell.用含有100μmol/L新福林的低渗台氏液灌流,ICl.swell电流强度增加.在-90 mV,电流强度由（-1.11±0.55）pA/pF增加到（-1.56±0.69）pA/pF（P＜0.05,n=7）;＋40mV,由（3.24±2.04）pA/pF增加到（4.64±2.61）pA/pF（P＜0.01,n=7）.这种效应为浓度依赖性,加入DIDS或者用等渗灌流液灌流,该效应被逆转.加入哌唑嗪1 μmol/L可以使新福林的量效关系曲线右移.结论 在人心房肌细胞新福林可以增强ICl.swell,这和在其他动物得到的结果不同.该现象是首次发现,表明新福林对ICl.swell的调节具有种属特异性.Objective To study the function of phenylephrine, an α1-adrenoceptor agonists, in the regulation of swelling-activated chloride current （ ICl.swell） in human atrial myocytes by a whole-cell patch-clamp technique. Methods Atrial myocytes were isolated from specimens of right atrial appendage obtained from patients undergoing coronary artery bypass and enzymatically dissociated. The myocytes were perfused with isotonic bath solution （1.0T） or hyposmotic bath solution （0. 6-times isosmotic, 0. 6 T）,and exposured to phenylephrine, 4,4＇-diisothiocyanostilbene-2,2＇ disulfonicacid （DIDS）, or prazosin, an α1-adrenoceptor antagonis respectively. The currents under different conditions were compared. Results ICl.swell evoked by hyposmotic bath solution was recorded. After approaching steady state values, subsequent exposure to 100 μmol/L phenylephrine caused enhancement of ICl.swell. The current was increased from （-1.11±0.55） pA/pF to （ - 1.56 ±0.69） pA/pF at -90 mV （P〈0.05,n=7） and from （3.24±2.04） pA/pF to （4.64±2.61） pA/pF at ＋40 mV （P〈0. 01,n=7） respectively. This effect was concentration-dependent, and could be reversed by DIDS or after exposure to the isotonic solution. Application of 1 μmol/L prazosin could attenuate the effect of phenylephrine. Conclusion Phenylephrine can enhance ICl.swell in human atrial myocytes, which is distinct from the reports in other species, indicating distinguished species-dependent variations in the modulation of ICl.swell, in atrial myocytes by phenylephrine.

关 键 词：氯离子电流 新福林 α1-肾上腺素受体激动剂 心房肌细胞 

分 类 号：R541[医药卫生—心血管疾病]