不同节段去交感神经对CCl4所致大鼠急性肝损伤的影响  

Effects of denervation of different sympathetic nerve segments on liver function in the carbon tetrachloride （ CCl4 ） -induced rats

作　　者：曾议[1] 夏锋[1] 田峰[1] 程龙[1] 王曙光[1] 

机构地区：[1]第三军医大学西南医院肝胆外科研究所,重庆400038

出　　处：《中华实验外科杂志》2010年第9期1262-1264,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30772095）

摘　　要：目的 观察不同节段去交感神经后对CCl4所致大鼠急性肝损伤的影响.方法 健康SD大鼠分别从颈交感干、内脏大神经和肝内行去交感处理,在此基础上应用CCl4制作急性肝损伤模型.检测各组肝功能、血清肿瘤坏死因子（TNF）-α及肝组织内去甲肾上腺素（NE）浓度.结果 在急性肝损伤的情况下,颈交感神经干离断和肝内去交感组31.80±1.50、31.74±1.08和假手术组29.90±1.20比较白蛋白升高,总胆红素降低（1.80±0.14、1.70±0.23、2.05±0.18,P＜0.05、P＜0.01）,肝组织内去甲肾上腺素水平下降（49.20 ±4.00、42.60±2.10、61.60±2.14,P＜0.01）.血清TNF-α较假手术组降低（192±18、318±20、398 ±40,P＜0.05）,而内脏大神经离断则无显著影响（476±30,P＞0.05）.结论 不同节段去交感神经可显著减轻CCl4所致急性肝损伤时的肝功能损害.交感神经不同节段激活在急性肝损伤早期有促进炎症效应.Objective To observe the effects of denervation of different segments of sympathetic nerve system on liver function in the carbon tetrachloride （CCl4） -induced rats. Methods Healthy SD rats were subjected to sympathetic denervation from cervical sympathetic trunk, greater splanchnic nerve and sympathetic nerve in liver. After 24 hours of acute liver injury model was established by using CCl4, Blood chemistry,tumor necrosis factor （TNF）-α, neuro-electrophysiology and the norepinephrinc （NE） concentrations of liver tissue were determined in each group. Results transection of cervical sympathetic trunk （TCST） and denervation of sympathetic nerve in liver group resulted in significant changes of parameters under acute liver injury, and has beneficial effects on protein anabolism （ 31.80 ± 1.50,31.74 ± 1.08,29.90 ± 1.20）, metabolism of bilirubin. （ 1.80 ± 0. 14, 1.70 ± 0. 23,2. 05 ± 0. 18, P 〈 0. 05, P 〈 0. 01 ）,but not transection of greater splanchnic nerve groups （ P 〉 O. 05 ）. Conclusion Sympathetic system has proinflammatory effect under acute liver injury due to CCl4.

关 键 词：肝损伤 去交感神经 去甲肾上腺素 肿瘤坏死因子 

分 类 号：R285.5[医药卫生—中药学]