HPLC法测定海龙中胸腺嘧啶、次黄嘌呤、尿嘧啶的含量  

Determination of thymine,hypoxanthine and uracil contents in medical pipefish by HPLC

作　　者：王梦月[1] 韦静斐[1] 史海明[1] 李晓波[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中国中药杂志》2010年第17期2277-2280,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672620)

摘　　要：目的：测定海龙药材中核苷类成分的含量,为其质量控制提供科学依据。方法：样品经蒸馏水超声提取后采用HPLC测定,Waters C18反相色谱柱,检测波长260 nm,柱温40℃,流动相0.05 mol.L-1KH2PO4-乙腈（97∶3）,流速0.6 mL.min-1,测定时间20 min。结果：胸腺嘧啶、次黄嘌呤、尿嘧啶分别在0.033~0.660（r=0.999 6）,0.620~12.400（r=0.999 9）,0.048~0.960μg（r=0.999 5）进样量与峰面积呈良好的线性关系;加样回收率分别为98.67%（RSD 1.6%）,99.03%（RSD0.74%）,98.65%（RSD 1.8%）。结论：建立了同时测定海龙中3种核苷类成分的方法,发现不同品种海龙中3种成分含量差异显著。本法快速、准确、灵敏,可用于海龙药材的质量控制。Objective： To establish a RP-HPLC method for simultaneous determination of thymine,hypoxanthine and uracil contents in medical pipefish.Method： Samples were extracted with distilled water by ultrasonic wave and separated on Waters C18 column eluted with a mobile phase of 0.05 mol·L-1 KH2PO4-acetonitrile（97∶3）.The flow rate was 0.6 mL·min-1.The determination wavelength was 260 nm and the column temperature was set at 40 ℃.Result： The method had good linearity in the range of 0.033-0.660（r=0.999 6）,0.620-12.400（r= 0.999 9）,0.048-0.960 μg（r= 0.999 5）,with average recoveries of 98.67%（RSD 1.6%）,99.03%（RSD 0.74%）,98.65%（RSD 1.8%）,for thymine,hypoxanthine and uracil respectively.Conclusion： The simultaneous determination method of thymine,hypoxanthine and uracil in medical pipefish is established by RP-HPLC for the first time.The contents of the three constituents in different kinds of medical pipefish are significantly different.The method is simple,rapid and sensitive,and can be used for control the quality of medical pipefish.

关 键 词：海龙 胸腺嘧啶 次黄嘌呤 尿嘧啶 含量测定 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]