GC-MS结合化学计量学方法用于女贞子乙醚提取物的定性定量分析  

Identification of chemical compositions in ether extracts of Ligustrum lucidum by GC-MS and chemometrics

作　　者：王向利[1] 张良晓[1] 梁逸曾[1] 戴慧[1] 

机构地区：[1]中南大学化学化工学院中药现代化研究中心,湖南长沙410083

出　　处：《中国中药杂志》2010年第17期2303-2307,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(20875104);科技部国际合作项目(2007DFA40680)

摘　　要：目的:对湖南产女贞子乙醚提取物化学成分进行定性与定量分析。方法:利用气相色谱-质谱(GC-MS)进行检测;通过直观推导式演进特征投影(HELP)法对产生的二维数据进行解析得到重叠峰中各组分的纯色谱图和质谱;并利用质谱相似度结合程序升温保留指数(PTRI),实现了对同分异构体(特别是构象异构)组分的定性分析,最后对各组分进行相对定量分析。结果:共分辨出84个色谱峰,鉴定了其中75个组分,占乙醚提女贞子化学成分总含量的84.3%。结论:女贞子主要挥发成分为顺式油酸(cis-oleic acid,9.805%)、羽扇豆醇(lupeol,8.848%)和9,12-亚油酸[(Z,Z)-9,12-octadecadienoic acid,8.357%]。Objective： To identify and quantify the compound compositions of ether extracts of Ligustrum lucidum.Method： After extracted 4 hour by Soxhlet extraction with ether,the extractive were analyzed by gas chromatography and mass spectrometry（GC-MS）.Furthermore,Heuristic Evolving Latent Projection（HELP） was used to resolve the overlapping peaks to obtain the pure concentration profiles and mass spectra.Subsequently,mass spectral similarity search combining with the retention index was employed for the identification of each component.Result： Totally,75 compounds were identified,accounting for 84.3% of that in the whole ether extracts of L.lucidum.Conclusion： The main compounds of L.lucidum are oleic acid,lupeol and（Z,Z） 9,12-octadecadienoic acid,whose relative content were 9.805%,8.848%,8.357%,respectively.

关 键 词：女贞子 乙醚提取 GC-MS 直观推导式演进特征投影法(HELP) 程序升温保留指数(PTRI) 

分 类 号：R284[医药卫生—中药学]