银杏酮酯GBE50对正常及模拟缺血豚鼠乳头肌动作电位的影响  

Effect of GBE50 on action potentials in normal and simulated ischemic guinea pig papillary muscles

作　　者：刘爱华[1] 张志雄[1] 

机构地区：[1]上海中医药大学生理教研室,上海201203

出　　处：《中国中药杂志》2010年第17期2342-2345,共4页China Journal of Chinese Materia Medica

基　　金：上海市教委重点学科项目(J50301);上海市教委科研项目(06cz017)

摘　　要：目的:观察银杏酮酯(GBE50)对正常及模拟缺血豚鼠乳头肌动作电位的影响,探讨GBE50的抗心肌缺血作用。方法:运用标准微电极细胞内记录技术观察GBE50对正常及模拟缺血豚鼠乳头肌动作电位参数(RP,APA,OS,APD20,APD50,APD90)的影响。结果:20,50,100 mg.L-1GBE50缩短心室乳头肌APD50和APD90(P<0.05),且呈剂量依赖性,而对其他参数没有影响;模拟缺血液灌流使RP,APA,OS分别减小,APD20,APD50,APD90分别缩短,而在缺血液中加入100 mg.L-1GBE50,缺血20 min仅APA缩短,其他参数无变化。结论:在生理状态下,GBE50可轻微缩短APD。在缺血状态下动作电位各项参数均减小,而GBE50通过维持缺血期间膜电位的稳定性,对抗缺血对跨膜电位的抑制效应,从而达到抗心肌缺血作用。Objective： To study the effect of Ginkgo biloba extract 50（GBE50）,a new multicomponent drug with a polyvalent action extracted from the leave of Ginkgo biloba,on the action potentials in normal and simulated ischemic guinea pig papillary muscles.Method： Standard intracellular microelectrode technique was used to examine the effects of GBE50 on the action potential parameters [action potential amplitude（APA）,overshoot,rest potential,action potential amplitude at 20%,50%,90% of repolarization（APD20,APD 50,APD90）].Result： In normal guinea pig cardiac papillary muscles,GBE50（20,50,100 mg·L-1） shortened APD50 and APD90,and did not affect the rest potential parameters.In simulated ischemic guinea pig cardiac papillary cells,action potential duration was significantly shortened,resting potential and action potential amplitude were reduced.100 mg·L-1 GBE50 partly attenuated the change induced by ischemia.Conclusion： GBE50 shortened APD of normal guinea pig cardiac papillary cells in a concentration-dependent manner.Under ischemia,all action potential parameters were reduced.GBE50 could alleviate the electrophysiological heterogeneity of ischemic myocardium,which may attenuate myocardial ischemia and block the onset of arrhythmia.

关 键 词：GBE50 缺血 乳头肌 动作电位 

分 类 号：R285.5[医药卫生—中药学]