7-硝基吲唑对在体大鼠海马l-黄皮酰胺和高频电刺激诱发长时程增强的影响(英文)  

Effects of 7-nitroindazole on long-term potentiation induced by l-clausenamide and high-frequency stimulation in rat hippocampus in vivo

作　　者：赵明瑞[1] 张均田[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所药理研究室

出　　处：《中国药理学报》1999年第4期319-323,共5页Acta Pharmacologica Sinica

基　　金：Project supported by the National Natural Science Foundation of China, № 29790121

摘　　要：目的:研究选择性nNOS抑制剂7-硝基吲唑(7-NI)在l-黄皮酰胺所致在体大鼠海马长时程增强(LTP)中的拮抗作用,方法:用细胞外记录技术记录麻醉大鼠齿状回中诱发动作电位的群峰电位(PS),结果:7-硝基吲唑(7-NI 2 nmol,icv)阻断高频电刺激(100 Hz)和l-黄皮酰胺(5 nmol,icv)诱导的LTP(n=6,P<0.01),L-精氨酸(225 mg·kg^(-1),ip)能逆转7-NI的这种作用(n=6,P<0.01),结论:nNOS产生的NO参与了l-黄皮酰胺所致海马LTP的诱导过程。AIM: To study the antagonistic effect of selective neuronal nitric-oxide synthase (nNOS) inhibitor 7-nitroindazole on the long-term potentiation (LTP) induced by l-clausenamide (Cla) in rat hippocampus in vivo. METHODS: Population spike (PS) of evoked potentials was determined by extracellular recording technique in the hippocampal dentate gyms (DG) of anesthetized rats. RESULTS: 7-Nitroindazole 2 nmol icv blocked the induction of LTP elicited by high-frequency (100 Hz) stimulation or Cla 5 nmol icv (P <0.01), and L-arginine 225 mg·kg-1 ip prevented the action of 7-nitroindazole (P < 0.01). CONCLUSION: Nitric oxide produced by nNOS plays a role in the induction of Cla-induced LTP in hippocampus.

关 键 词：吲唑类 一氧化氮合酶 黄皮酰胺 长时程增强 海马 

分 类 号：R338.64[医药卫生—人体生理学] R964[医药卫生—基础医学]