商洛多花胡枝子根瘤菌生理生化特性和数值分类研究  

Study on physiological and biochemical characters and numerical taxonomy of rhizobia isolated from Lespedeza floribunda in Shangluo Region of Shaanxi

作　　者：冀玉良[1,2] 詹俊鹏[3] 韦革宏[3] 

机构地区：[1]商洛学院生物医药工程系,陕西商洛726000 [2]西北农林科技大学生命科学学院,陕西省农业分子生物学重点实验室,陕西杨凌712100 [3]西北农林科技大学生命科学学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《干旱地区农业研究》2010年第5期97-103,共7页Agricultural Research in the Arid Areas

基　　金：陕西省教育厅科学技术研究项目(08JK280)

摘　　要：选用分离自陕西商洛多花胡枝子的59株根瘤菌和15株参比菌,进行了125项表型性状测定和聚类分析。结果表明:未知菌能够利用的碳氮源比较广泛,生长的温度和pH范围较宽,对抗菌素、染料和重金属分别具有抗性的菌株以及产氧化酶菌株的数量比例较高,部分菌株具有耐高浓度盐或碱的性能,其中大约67%的菌株能耐受3.0%的NaCl,25%的菌株可在初始pH12的YMA培养基上生长。从数值分类树状图谱发现,在80.5%的相似性水平上,除CCNWSX0922外,供试菌株聚成8个表观群,群Ⅰ包含6株未知菌和3株已知中慢生根瘤菌属参比菌,群Ⅱ包含19株未知菌和6株中华根瘤菌属参比菌,群Ⅲ包含1株未知菌和3株根瘤菌属参比菌,群Ⅳ和群Ⅴ皆含根瘤菌属参比菌株。群Ⅵ、群Ⅶ和群Ⅷ三个类群未能与参比菌株聚在一起,为新类群,其分类地位需进一步研究确定。59 unknown rhizobia strains isolated from Lespedeza floribunda in Shangluo,Shaanxi Province and 15 reference strains were analyzed with 125 phenotypic features and numerically classified.The result showed that the unknown strains could use the majority of all the carbn and nitrogen sources and adapt to a wide range of temperature and pH.The proportion of unknown strains which could be resistant to antibiotic,dye and heavy metals and could produce oxidase was relatively high.Some strains had endurance property to high concentrations of salt or alkali,in which about 67% strains could be resistant to 3.0% NaCl and 25% strains could grow in YMA medium with pH12.Numerical taxonomy showed that,in addition to strain CCNWSX0922,other test strains were clustered into 8 clusters at 80.5% similarity level.Clusters Ⅰ contained 6 unknown strains and 3 reference strains of Mesorhizobium.Cluster Ⅱ had 19 unknown strains and 6 reference strains of Sinorhizobium.Cluster Ⅲ consisted of 1 unknown strain and 3 reference strains of Rhizobium.Cluster Ⅳ and Ⅴall included reference strains of Rhizobium.Clusters Ⅵ,Ⅶ and Ⅷ may be new groups,which were not clustered into the reference strains,and their taxonomic status must be confirmed by further measurements.

关 键 词：多花胡枝子 根瘤菌 表型多样性 

分 类 号：S144.3[农业科学—肥料学]