棉花三种育苗移栽新方法缓苗期棉苗若干生理生化的比较  

Study on Physiological and Biochemical Indicators of Seedling of Three New Seedling-raising and Transplanting Methods at Recovering Stage

作　　者：刘小玲[1] 毛树春[1] 韩迎春[1] 李亚兵[1] 范正义[1] 王国平[1] 冯璐[1] 董春旺[1] 朱巧玲[1] 

机构地区：[1]中国农业科学院棉花研究所/农业部棉花遗传改良重点开放实验室,河南安阳455000

出　　处：《棉花学报》2010年第5期437-442,共6页Cotton Science

基　　金：农业部公益性行业专项(3-5);国家科技支撑计划(2007BAD44B03);公益性院所长基金(SJA0908);国家棉花产业技术体系等

摘　　要：2009年春秋2次试验旨在比较基质育苗裸苗移栽、水浮育苗带基质移栽和无土育苗无钵移栽3种方法缓苗期棉苗生理生化指标的差异。2次试验育苗均在温室进行,春季试验幼苗移栽大田采用完全随机区组设计,秋季采用微区设计,均为3次重复。结果表明:3种育苗方法2次试验均因移栽时产生的逆境胁迫而使叶绿素含量、MDA含量在缓苗期内呈现先急剧升高而后迅速下降再趋于平稳的变化规律。缓苗期内SOD、POD、CAT三种酶活性均为先上升后下降再稳定于某个水平,且三者呈现一定的相关性。基质苗从缓苗后期开始,新根生长迅速且在取样结束前总长高于无钵苗,低于水浮苗,但三者无显著性差异。基质苗的干重和叶面积介于水浮苗与无钵苗之间。This study was conducted in spring and fall of 2009 to investigate three new seedling-raising methods（substrate seedling-raising and bare-root transplanting,floating seedling-nursing in nutrient water-bed and transplanting with substrate,as well as soilless seedling-raising and transplanting without carrier） through comparing the physiological and biochemical indica-tors of the seedlings at recovering stage.Seedlings were nursed in greenhouse.Experiment design was a randomized complete blocks in spring,and a micro-area design in autumn with three replications,respectively,in the field.The results showed that chlorophyll content,MDA content in seedlings of the three methods increased sharply due to the stress,and then decreased rapidly until a certain level and kept steady since then.Similarly,enzymatic activity of SOD,POD,CAT increased at the begin-ning and then decreased to a certain level and stayed stable.SOD activity changed relatively mildly comparing to that of POD and CAT,and enzymatic activity of the three methods showed a correlative relation.The dry weight,leaf area,and new root length increased along with the reproductive process.Moreover,after recovering stage,new roots of substrate seedling-raising method grew quickly and turned longer than that of soilless seedling-raising method,while less than that of floating seedling-raising method.But there were no significant difference in statistics.The dry weight and leaf area regarding seedlings of substrate seedling-raising method were between the other two methods.

关 键 词：棉花 育苗新方法 缓苗期 生化指标 

分 类 号：S562.048[农业科学—作物学]