转AhCMO基因棉花苗期对干旱胁迫的生理反应  

Physiological Responses of AhCMO Transgenic Cotton Seedlings to Water-Deficit

作　　者：朱玉庆[1,2] 罗振[1] 张慧军[3] 孔祥强[1] 陈受宜[4] 董合忠[1] 孙学振[2] 

机构地区：[1]山东省农业科学院棉花研究中心,济南250100 [2]山东农业大学农学院,山东泰安271018 [3]山西省农业科学院棉花研究所,山西运城044000 [4]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《棉花学报》2010年第5期443-447,共5页Cotton Science

基　　金：转基因生物新品种培育科技重大专项(2009ZX08005-018B);国家自然科学基金(30971720);山东省农业良种工程重大课题(2009LZ005-05)

摘　　要：以转AhCMO基因的2个棉花品系L1和L2及其转化受体泗棉3号(SM3)为材料,研究了转AhCMO基因棉花对干旱胁迫的生理反应。试验采用盆栽方式在日光温室中进行,以维持土壤含水量为最大持水量的45%作为干旱处理,以正常供水维持土壤含水量为最大持水量的75%作为对照。结果表明,正常供水条件下转基因品系L1和L2与SM3生长表现一致。但是干旱胁迫下,转基因品系L1和L2的干物质积累量、平均净光合速率以及叶片叶绿素的含量都显著高于SM3;而且L1和L2叶片中甜菜碱含量显著高于SM3,过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性也较SM3显著提高。说明2个转AhCMO基因品系的耐旱性得到明显提高,耐旱性的提高与甜菜碱积累量的增加、POD和SOD活性的增强有关。Water-deficit treatment was conducted on two AhCMO transgenic cotton lines（L1 and L2） and a non-transgenic cv.Simian 3（SM3） to study the physiological responses in seedling stage of AhCMO transgenic cotton,by using pots filled with fertile soil in a green house.Soil moistures were maintained 45% and 75% of the soil water capacity as the drought-stressed treatment and the non-stressed control,respectively.No obvious morphological or developmental differences existed between the transgenic lines（L1 and L2） and field plants in the absence of drought stress.The contents of glycine betain,activities of antioxidant enzymes（POD and SOD）,the biomass,net photosynthesis rates and leaf chlorophyll content of transgenic AhCMO cotton lines（L1 and L2） were observably higher than those of SM3 at 20 d after stress treatment had been applied.It is suggested that the enhanced accumulation of glycine betain and the increase of the activities of antioxidant enzymes（POD and SOD） improved water-deficit tolerance in transgenic cotton plants.

关 键 词：棉花 AhCMO基因 干旱胁迫 生理反应 

分 类 号：S562[农业科学—作物学]