铁皮石斛总生物碱含量变异规律  

Quantitive variation of total alkaloids contents in Dendrobium officinale

作　　者：诸燕[1] 张爱莲[1] 何伯伟 张新凤[1] 俞巧仙 斯金平[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地天然药物研发中心,浙江临安311300 [2]浙江省中药材产业协会,浙江杭州310020 [3]浙江森宇药业有限公司,浙江义乌322000

出　　处：《中国中药杂志》2010年第18期2388-2391,共4页China Journal of Chinese Materia Medica

基　　金：浙江省重大林业科技项目(2007A12);浙江省重大科技专项(2009C12059)

摘　　要：目的:全面了解不同种质、不同采收年限铁皮石斛药材总生物碱含量及其变异规律,为铁皮石斛真伪鉴别与优质药材培育提供依据。方法:采集铁皮石斛人工栽培骨干基地一至三年生铁皮石斛17个种质41个样品,以及市售不同价位枫斗药材11个样品;采用酸性染料比色法测定总生物碱含量。结果:人工栽培的铁皮石斛与市场购买的铁皮枫斗药材总生物碱在0.019 0%～0.043 0%;浙江省11个铁皮石斛种质一年生、二年生、三年生样品平均总生物碱为0.025 3%,0.027 0%,0.032 6%,云南产一年生铁皮石斛平均总生物碱为0.034 3%。结论:不同种质、年龄的铁皮石斛总生物碱含量差异很大,并且总生物碱含量随着生长年限的增加而增加,云南产铁皮石斛总生物碱明显高于浙江产铁皮石斛,但正宗的铁皮石斛总生物碱含量具有明显限量范围,总生物碱含量明显偏离限量范围的样品可以基本确定为非铁皮石斛,总生物碱含量在此范围的样品必须结合多糖含量与其他活性化学组分才能真正辨别铁皮石斛药材的真伪与优劣。Objective： To investigate the total alkaloid content and find out the variation of total alkaloids content in Dendrobium officinale with different germplasms and harvesting ages with the aim of providing scientific basis for the authenticity identification and the breeding of D.officinale.Method： Forty one samples with 1-3 ages were collected from cultivated fields of Zhejiang and Yunnan provinces and 11 samples were collected from markets.The total alkaloid contents of samples were determined using acid-dye colorimetry method.Result： The determined total alkaloid contents in D.officinale ranged 0.019 0%-0.043 0%.The averages of total alkaloid contents were 0.025 3%,0.027 0% and 0.032 6% in 1-year-old,2-year-old and 3-year-old samples of Zhejiang province,respectively.The average of total alkaloid content was 0.034 3% in 1-year-old samples of Yunnan province.Conclusion： The total alkaloid content of D.officinale with different germplasms and physiological ages existed significant discrepancy,and it increased with the growth of ages.The total alkaloid content of cultivated D.officinale in Yunnan province was higher than that in samples from Zhejiang province.But the authentic D.officinale had certain range of total alkaloid content.Samples whose total alkaloids content divagates from this range must be counterfeit;Samples whose total alkaloid content is in this range still need to combines with other active chemical groups like polysaccharides content to identify the authenticity and quality of medicinal materials.

关 键 词：铁皮石斛 种质 生理年龄 总生物碱 酸性染料比色法 

分 类 号：S567.239[农业科学—中草药栽培]