清开灵有效组分调节小鼠海马缺血相关基因的共性与多样性分析  

Similarity and diversity analysis of Qingkailing effective components in regulating hippocampus ischemia-related genes of mice

作　　者：周才秀[1,2] 王忠[1] 张占军[3] 荆志伟[1] 

机构地区：[1]中国中医科学院中医临床基础医学研究所,北京100700 [2]湖南省株洲市田心医院,湖南株洲412000 [3]北京师范大学认知神经科学与学习国家重点实验室,北京100875

出　　处：《中国中药杂志》2010年第18期2475-2479,共5页China Journal of Chinese Materia Medica

基　　金：国家“十一五”科技支撑计划项目(2006BAI08B04-06)

摘　　要：目的:比较清开灵组分黄芩苷(BA),栀子苷(JA),胆酸(CA)与珍珠母(CM)在治疗海马缺血过程中基因表达变化机制。方法:75只6周龄小鼠随机分为BA,JA,CA,CM,M(模型组),每组15只,建立海马缺血再灌注小鼠模型,2 h后实验组分别经尾静脉按3 mL.kg-1体重注射相应药物,24 h后断头取脑制作冠状切片,TTC(氯化四唑)染色,计算不同组别梗死体积百分比;同时抽提小鼠海马组织的总RNA,利用与脑缺血相关的374个基因的cDNA芯片检测基因表达谱变化,采用Ar-raytrack软件选取P<0.05,Fold change>1.5的差异基因,根据差异基因的GO功能探讨药效作用特点。结果:除CM组外,BA,JA,CA与模型组比较均能有效减少海马缺血梗塞面积(P<0.05)。BA,JA,CA与CM比较差异基因数量分别为41条(上调24,下调17),22条(上调13,下调9),11条(上调8,下调3),它们共同抑制Myb基因的表达。结论:BA,JA,CA产生药效结果不仅有共同的基因作用靶点和作用机制,同时还存在多样性的特点。Objective： To compare the different gene expression profiles among Qingkailing components of BA（baicalin）,JA（jasminoidin）,CA（cholic acid）and CM（concha margaritiferausta）in regulating hippocampus ischemia related genes of mice.Method：The hippocampus ischemia-reperfusion model mice were randomly divided into groups of BA,JA,CA,CM and M（model group）,15 mice for each group,and decapitated after 24 hours.Coronal brain slices were stained with TTC（2,3,5-Triphenylte trazolium Chloride） and the percentages of infarct volume were calculated.Meanwhile,total RNA were extracted from the hippocampus.We selected 374 genes which related to cerebral ischemia to find the different gene expression profiles among the Qingkailing components.Then T-tests was used to select different genes between BA and CM,JA and CM,CA and CM by Arraytrack software（P〈0.05,Fold change1.5）,and the pharmacodynamic characteristics were explored according to GO functional classification.Result： Compared with the model group BA,JA and CA could effectively reduce infarct size of hippocampus ischemic（P〈0.05）.the numbers of significantly differentially expressed genes were 41（24 up,17 down） between BA and CM,22（13 up,9 down）between JA and CM,and 11（8 up,3 down）between CA and CM.All of BA,JA and CA could inhibit the expression of Myb gene.Conclusion： When exerting its pharmacological effects,BA,JA,CA not only have common gene targets but also have diversity in pharmacological character.

关 键 词：清开灵 脑缺血 基因功能 

分 类 号：R285.5[医药卫生—中药学]