半夏遗传结构和多样性RAPD标记分析  

Genetic Structure and Diversity among Pinellia ternata Revealed by RAPD Markers

作　　者：张君毅[1] 

机构地区：[1]华侨大学,福建厦门361021

出　　处：《中国农学通报》2010年第18期43-47,共5页Chinese Agricultural Science Bulletin

基　　金：华侨大学人才引进项目"半夏传多样性研究"(2008BS103);福建省自然科学基金项目"短葶山麦冬遗传多样性评价及质量控制"(2009J05078)

摘　　要：为保护和利用半夏种质资源,利用RAPD标记技术对取自半夏主要分布区16个省的24份种质进行遗传多样性分析。结果表明:在物种水平上,多态位点百分率(PPL)68.77%,Nei’s基因多样性指数(He)0.2062;居群水平上,PPL为9.88%～58.02%,平均27.21%,群体间遗传分化系数(Gst)0.5282,居群间基因流(Nm)0.4466。UPGMA聚类分析显示,当相似系数0.896时,24份种质可分为5个类群。To protect and utilize germplasm resources ofPinellia ternate , random amplification of polymorphic DNA （RAPD） marker was occupied to study the genetic diversity of 24 germplasm from 16 main distribution provinces. The results showed that at species level polymorphic loci percentage （PPL） is 68.77%, Nei’s gene diversity index （He） 0.2062. At population level, PPL varied from 9.88% to 58.02%, with the average value 27.21% and groups of genetic differentiation factor （Gst） is 0.5282, population gene flow （Nm） 0.4466. The results of cluster analysis based on UPGMA revealed that when similar coefficient was 0.896, 24 populations could be divided into five material groups.

关 键 词：半夏 RAPD标记 遗传多样性 遗传结构 

分 类 号：R931.2[医药卫生—生药学]