离子束法导入大豆DNA小麦的蛋白质含量分析  

Protein Content Analysis of the ion Bean-Mediated Soybean DNA Transferred Wheat

作　　者：苌收伟 秦广雍[2] 余增亮[3] 吴跃进[3] 程玉红 苏明杰[2] 

机构地区：[1]河南省鹤壁市农科所,河南鹤壁458030 [2]郑州大学离子束生物工程省重点实验室,郑州450052 [3]中国科学院离子束生物工程学重点实验室,合肥230031

出　　处：《中国农学通报》2010年第18期71-74,共4页Chinese Agricultural Science Bulletin

基　　金：国家"十五"科技攻关项目(2001BA302B-03);中国科学院离子束生物工程学重点实验室开放基金资助项目(2004B007)

摘　　要：为了提高小麦蛋白质含量,采用离子束介导法将'豫豆23号'(Glycine max)的基因组DNA导入普通小麦(Triticum aestivum L.)品种'中育5号'和'淮阴9628',获得了114株T1代高蛋白含量(>15%)单株,蛋白质含量最高达21.44%,显著提高了受体亲本小麦蛋白质含量。从T1到T3代连续单株选择,获得了16个遗传上基本稳定的T3代株系。结果表明,离子束介导大豆DNA导入小麦,可显著提高转化后代株系的蛋白质含量。随着世代的提高群体蛋白含量有逐步提高的趋势,在T3代基本形成遗传稳定的高蛋白品系。该方法是小麦蛋白质含量改良的可行途径。In order to improve wheat （Triticum aestivum L.） seed protein content, soybean （Glycine max ） genomic DNA of‘Yudou 23’was transferred into two wheat varieties‘Zhongyu 5’and‘Huaiyin 9628’by low energy ion beam-mediated transformation. Total 114 individuals with high protein contents （15%） of the first generation were obtained, the top value was 21.44%. The protein contents of soybean DNA transferred wheat individuals were significantly increased compared to their parents. Total 16 near stably inherited lines with high protein contents were obtained by individual selection from the first generation to the third generation. The result showed that low energy ion beam-mediated transformation of soybean genomic DNA into wheat could significantly increase the protein content of the transferred lines. The protein contents of transferred lines were increased with propagating, and they were near stable at the third generation. The method in this paper is an efficient pathway for wheat protein improvement breeding.

关 键 词：小麦 离子束 大豆 DNA 蛋白质 

分 类 号：S332[农业科学—作物遗传育种]