富铁苹果发酵液对缺铁胁迫下平邑甜茶保护酶活性的影响  

Studies on the Effects of Iron-rich Apple Fermentation Broth on Protective Enzymes Activities of Malus hupehensis under Iron-deficiency Stress

作　　者：戚亚平[1] 王荣娟[1] 姚允聪[1] 姬谦龙[1,2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物技术学院,北京102206

出　　处：《中国农学通报》2010年第18期201-205,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑重大项目"主要果树高效标准生产技术"(2008BAD92B08);北京市农委资助项目(20070624);北京农学院基础科学基金资助项目(062ZD2JW205)

摘　　要：为探明不同浓度苹果果实发酵液加铁对缺铁平邑甜茶(M.hupehensis Rehd)保护酶活性的影响。以缺铁平邑甜茶为试材,用加铁苹果果实发酵液进行根施恢复试验。结果表明:对一年生平邑甜茶幼苗,T3(稀释300倍发酵液+FeSO4)处理的CAT、POD的活性和MDA、可溶性糖含量分别较T1(去离子水对照)处理高了283.6%、58.2%、271.8%、50.3%,T3处理的4个指标均显著高于其他处理;可溶性糖含量除外,T2(稀释100倍发酵液+FeSO4)处理比T4(稀释500倍发酵液+FeSO4)处理和T5(FeSO4溶液)处理的作用显著;对于三年生平邑甜茶/长富2号缺铁苹果树来说,A3(稀释300倍发酵液+FeSO4)处理的CAT、POD的活性和MDA、可溶性糖的含量分别比A1(浇去离子水对照)处理高了400.0%、88.3%、39.4%、48.4%,A3(稀释300倍发酵液+FeSO4)处理较A2(FeSO4溶液)处理作用显著。总体上,稀释300倍苹果果实发酵液+FeSO4对保护酶活性影响显著高于其他处理,恢复效果最好。To study the effects of different fold diluted apple fermentation broth＋FeSO4 on protective enzymes activities of iron-deficiencyMalus hupehensis. Iron-deficiencyMalus hupehensis seedlings cultured in sand were watered with the iron-rich apple fermentation broth to recover the iron-deficiency phenomenon. The results showed that： For Malus hupehensis seedlings, compared with T1 （deionized water） treatment, the activities of CAT, POD and contents of MDA, soluble sugar of T3 （diluted 300-fold fermentation broth ＋FeSO4） treatment increased 283.6%, 58.2%, 271.8%, 50.3%, respectively. The four indexes of T3 treatment was also higher than the other four treatments significantly. Except the soluble sugar, the effect of T2 （diluted 100-fold fermentation broth ＋FeSO4） treatment was better than T4 （diluted 500-fold fermentation broth ＋FeSO4） and T5 （FeSO4） treatment significantly. For three-years iron-deficiency apples, compared with A1 （deionized water） treatment, the activities of CAT, POD and contents of MDA, soluble sugar of A3（diluted 300-fold fermentation broth ＋FeSO4） treatment increased 400.0%, 88.3%, 39.4%, 48.4% respectively. The effect of A3 （diluted 300-fold fermentation broth ＋FeSO4） was better than A2 （FeSO4） treatment significantly. As a whole, 300-fold diluted fermentation broth ＋FeSO4 was the best treatment for both iron-deficiency seedings and three-years iron-deficiency apples.

关 键 词：苹果果实发酵液 平邑甜茶 缺铁胁迫 保护酶 

分 类 号：S661.1[农业科学—果树学]