中国对虾幼体中肠的超微结构  

ULTRASTRUCTURE OF THE MIDGUT IN THE LARVAE OF PENAEUS CHINENSIS

作　　者：张志峰[1] 马爱军[2] 于利[1] 廖承义[1] 

机构地区：[1]青岛海洋大学海洋生命学院,青岛266003 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《海洋与湖沼》1999年第2期144-149,共6页Oceanologia Et Limnologia Sinica

基　　金：国家攀登计划B资助!PD B-6-2-2

摘　　要：1995年4月，在山东日照市涛雒对虾育苗场采集中国对虾各期幼体，采用超薄切片和电镜观察的方法，对其中肠结构进行观察研究。结果表明，中国对虾幼体的中肠肠壁由3部分组成，即肠上皮、基膜和肌层。中肠上皮细胞具发达的微绒毛，胞质中富含线粒体、内质网、高尔基复合体及酶原颗粒等，它由两类细胞（含脂肪颗粒的细胞和不含脂肪颗粒的细胞）组成。第一类细胞在发有早期占多数，随着幼体的发育逐渐减少，至仔虾期后，肠上皮完全由第二类细胞组成。基膜分为内、外两层，且在各期中基本无变化。肌层分为环、纵肌层，在发育早期分层不明显，但肌层随着幼体的发育逐渐发达。Larval Penaeus chinensis (from nauplius topost larvae) were collected from Taoluo Ihawn Breeding Fastory, Rjzhao City, Shandong Province, in April 1995. The dridgut ultrastrucare was observed by TEM. The larval Penaeus chinensis samples were prefixed in 2.5% glutaraldehyde and postfixed in 1% osmic acid. After dehydrihon in a series of graded ethanol, they were transfermd into acetone and embedded in Epon-812. After sitting by the sedri-thin section technique, the ultra-thin sections were stained with lead citrate and uranyl acetate. The result shows that midgut is most developed in the digeshve tube. It consists of three parts, i.e. midgut epithelium, basal membIane and muscular layers. Epithelial cells have abundant microvilli and contrin rich mitochondria, endoplasmic reticulum, Golgi complex and zymogen granules in cytoplasm. Two epithelial cell types can be dishnginshed: the cells conhaning liPid granules (Flg. 1. 1) and the cells lacking lipid granules (Flg. 1:3). The main cell type is the first one at the eariy stage, but in the development process, the first type ceIls become fewer gfadually, and disaPpear at the postlarval stage. Eventually, the ePithelium oniy consistS of the second type cells. The first type cell is similar to the B-cell of the Andgut gland in the strUcfor (Wang et al, 1997), with lipid granules in it.The lipid granules change from many small ones to a few large ones. At last,the large lipid gnmules are released into the midgut lum. The simildrity in structure further confirms that the whdgut gland is from the midgut and the B-cell is from the first type cell of the nddgut at firsL Because the B-cell partjcipates in cytcrdigeshon in nddgut gland (Al-Mohanna et al, 1986), at the eary stage the Andgut epithelium pAncipates in absorPhon and digeshon before the midgut gland developed. Zymogen and 1ipid gfanules of the first type cells are mainiy used in digeshon. With the gradual nddgutgland development the digeshve funchon of the whdgut is replaced by the midgut gland's. At the mysis stag

关 键 词：中国对虾 幼体 中肠 超微结构 

分 类 号：Q959.223.5[生物学—动物学] S968.226[农业科学—水产养殖]