豌豆根瘤菌共生质粒pJB5JI对费氏中华根瘤菌共生固氮作用的影响  

Effect of the symbiotic plasmid pJB5JI of Rhizobium leguminosarum. bv. viciae on the symbiotic nitrogen fixation of Sinorhizobium fredii

作　　者：杨成运[1,2] 樊剑鸣[1] 张书杰[3] 周俊初[2] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450000 [2]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [3]郑州大学生物工程系,河南郑州450000

出　　处：《河南农业大学学报》2010年第4期462-467,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30470065);科技部微生物学资源课题(2005DKA21208-6)

摘　　要：通过两亲本接合转移,将豌豆根瘤菌T83K3共生质粒pJB5JI转入费氏中华根瘤菌HN01及其质粒缺失突变株中,获得8个转移接合子,其中受体为HN01和pSfrHN01a缺失突变株HND28的接合子能够在大豆上形成有效根瘤,无论是人工传代还是与植物共生,外源质粒pJB5JI与受体菌内源质粒均能稳定存在,但不能在豌豆上结瘤,与大豆共生固氮能力和竞争结瘤能力与受体菌亲本相比没有明显变化.消除共生质粒的突变株HND29和共生质粒与pSfrHN01a质粒均消除的突变株HND100作受体菌的接合子,既不能在大豆上结瘤,也不能在豌豆上结瘤.研究结果表明,pJB5JI与HN01的3个质粒属于不同的不相容群.pJB5JI在HN01中的表达功能可能受到受体菌的共生质粒和染色体基因的共同影响.The symbiotic plasmid pJB5JI of Rhizobium leguminosarum.bv.viciae T83K3 was transferred into Sinorhizobium fredii HN01 and its plasmid cured derivatives.Eight transconjugants were obtained.The transconjugants of HN01 and HND28 could form the effective nodules on soybean.The exogenous plasmid pJB5JI and endogenous plasmids of HN01 were stable under both free-living conditions and symbiosis.But they failed to nodulate pea.The ability of competition and symbiotic nitrogen fixation of the conjugants could not significantly change compared with the recipients.The conjugants of HND29 and HND100 failed to nodulate soybean and pea.The above results indicated that pJB5JI and the three plasmids of HN01 belonged to different incompatible group.The express of pJB5JI function was co-affected by the symbiotic plasmid and chromosome of recipients.

关 键 词：共生质粒 费氏中华根瘤菌 pJB5JI 质粒转移 

分 类 号：Q939.96[生物学—微生物学]