10-23脱氧核酶对CTX-M-38型超广谱β-内酰胺酶的抑制作用  

Inhibitory effects of 10-23 deoxyribozyme on CTX-M-38 gene expression in E. coli

作　　者：张志坚[1] 郭小兵[2] 李海霞[3] 张钦宪[4] 

机构地区：[1]郑州大学第三附属医院检验科,郑州450052 [2]郑州大学第一附属医院检验科,郑州450052 [3]河南省人民医院检验科,郑州450003 [4]郑州大学基础医学院组织学与胚胎学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2010年第5期740-742,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关基金资助项目200703071

摘　　要：目的:探讨10-23脱氧核酶(10-23DRz)抑制CTX-M-38型ESBLs的可行性。方法:依据CTX-M-38型ESBLs序列及其mRNA构象特征,设计针对其mRNA结构的10-23DRz脱氧核酸序列及反义核酸序列;采用氯化钙法对10-23DRz及反义核酸进行转化;依据转化菌在平板上菌落形成状况及菌液吸光度选择10-23DRz应用量;采用K-B法体外药敏试验检测耐药活性。结果:45nmol10-23DRz用量时转化菌菌落形成量及菌液吸光度较为理想。除哌拉西林、头孢噻肟及亚胺培南外,其他抗菌药物10-23DRz组与反义核酸组抑菌环直径间差异有统计学意义(P均<0.001)。结论:10-23DRz可抑制CTX-M-38型ESBLs的表达。Aim：To research the inhibitory effects of 10-23 deoxyribozyme（10-23DRz） on CTX-M-38 gene expression in E.coli.Methods：According to feature of the CTX-M-38 type ESBLs gene sequence and mRNA structure,10-23DRz and antisense oligonucleotides were designed.And the designed products were introduced into BL21-pET-CTX-M-38 respectively by the CaCl2 procedure,applied concentration was selected by colony number and A（600 nm） value.The inhibitory effects was observed by K-B discagar diffusion test.The result was analyzed by chi square test.Results：The applied concentration of 10-23DRz was 45 noml.Except piperacillin,imipenem and cefotaxime,the deviation between the 10-23DRz group and antisense oligonucleotides group was significant（P0.001）,indicated that the inhibitory effects of 10-23DRz is better than antisense oligonucleotides and corresponding group.Conclusion：10-23DRz could specifically inhibit the CTX-M-38 type ESBLs expression in BL21-pET-CTX-M-38.

关 键 词：10-23脱氧核酶 CTX-M-38型 超广谱Β-内酰胺酶 

分 类 号：R446.5[医药卫生—诊断学]