超高效液相色谱-串联质谱法测定牛奶中的氯霉素残留  

Determination of Chloramphenicol Residues in Milk by UPLC-MS/MS Method

作　　者：栗旸[1,2] 和顺琴[2] 薛景娇[2] 曹靖丽[2] 胡秋芬[2] 

机构地区：[1]云南省卫生防疫站疾控中心,云南昆明650022 [2]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《畜牧与饲料科学》2010年第8期91-92,182,共3页Animal Husbandry and Feed Science

基　　金：云南民族大学重点实验室基金;云南民族药资源委部共建重点实验室开放课题资助

摘　　要：研究了用超高效液相色谱-串联质谱法测定牛奶中的氯霉素。牛奶样品先用高氯酸沉淀蛋白,再用乙酸乙酯匀浆法提取样品中的氯霉素。样品经净化后以乙腈-水作为流动相,C18超高效液相色谱柱为固定相分离氯霉素,然后用电喷雾质谱负离子监测模式对氯霉素进行检测。该方法避免了常规液相色谱法会出现的假阳性现象,还大大缩短了色谱分离时间,提高了检测灵敏度,用于实际样品分析,其结果令人满意。Chloramphenicol residues in milk were detected by UPLC-MS/MS.The protein in milk was first precipitated by perchloric acid,and chloramphenicol in milk was extracted by homogenization with ethyl acetate.After purification,the chloramphenicol was separated by high performance liquid chromatography with C18 column as stationary phase and acetonitrile-water as mobile phase.The chloramphenicol residues were detected by MS/MS in the ESI（-）-MRM mode.This method can avoid the false positive results of routine HPLC,largely reduces separation time,and improves sensitivity.It can be used detect chloramphenicol in milk,and the detection is satisfactory.

关 键 词：氯霉素 牛奶 超高效液相色谱-串联质谱法 测定 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]