Effects of Bushenyiqihexue Formula on the Endometrial Gland Apoptosis in Mice with Blastocyst Implantation Dysfunction  

作　　者：黄冬梅 黄光英 陆付耳 周永生 

机构地区：[1]Institute of the Integration of Traditional Chinese and Western Medicines, Tongji Medical College of Huazhong University of Science and Technology

出　　处：《Journal of Traditional Chinese Medicine》2010年第3期196-200,共5页中医杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30171193)

摘　　要：Objective: To observe the effects of Bushenyiqihexue Formula (补肾益气和血方 Formula for Tonifying the Kidney, Replenishing qi and Harmonizing Blood, FTKRQHB) on the endometrial gland apoptosis in the mice with blastocyst implantation dysfunction. Methods: The mice with the first-day pregnancy were divided into the control, model and treatment groups, with 30 in each group, and blastocyst implantation dysfunction was induced by subcutaneous injection of mifepristone in the mice of the model and treatment groups. The pregnancy rate and implantation number of blastocysts were measured and the expressions of proliferating cell nuclear antigen (PCNA), Bax, Bcl-2, and activated caspase-3 were detected in all the three groups. Results: The model group had significantly depressed pregnancy rate, implantation number of blastocysts and apoptosis index, and elevated proliferation index of endometrial gland as compared with the control group (P<0.05 or P<0.01). Administration of FTKRQHB (the treatment group) resulted in significant increases in pregnancy rate, implantation number of blastocysts and apoptosis index of the endometrial gland, and a significant decrease in the proliferation index of the endometrial gland as compared with the model group (P<0.05 or P<0.01). The differences in the four indexes between the treatment group and control group were not significant statistically. The Bax and activated caspase-3 expressions in endometrial gland in the model group became significantly lower than that of the control group (P<0.01), whereas those in the treatment group were significant higher than that of the model group (P<0.01). However, the Bax and activated caspase-3 expressions in endometrial gland were similar in both treatment and control groups. Conclusion: Promoting the increases in Bax and activated caspase-3 expressions in the endometrial gland and bringing into balance between apoptosis and proliferation of the glandular cells at the implantation window phase by FTKRQHB may contribute to the effects of pObjective： To observe the effects of Bushenyiqihexue Formula （补肾益气和血方 Formula for Tonifying the Kidney, Replenishing qi and Harmonizing Blood, FTKRQHB） on the endometrial gland apoptosis in the mice with blastocyst implantation dysfunction. Methods： The mice with the first-day pregnancy were divided into the control, model and treatment groups, with 30 in each group, and blastocyst implantation dysfunction was induced by subcutaneous injection of mifepristone in the mice of the model and treatment groups. The pregnancy rate and implantation number of blastocysts were measured and the expressions of proliferating cell nuclear antigen （PCNA）, Bax, Bcl-2, and activated caspase-3 were detected in all the three groups. Results： The model group had significantly depressed pregnancy rate, implantation number of blastocysts and apoptosis index, and elevated proliferation index of endometrial gland as compared with the control group （P〈0.05 or P〈0.01）. Administration of FTKRQHB （the treatment group） resulted in significant increases in pregnancy rate, implantation number of blastocysts and apoptosis index of the endometrial gland, and a significant decrease in the proliferation index of the endometrial gland as compared with the model group （P〈0.05 or P〈0.01）. The differences in the four indexes between the treatment group and control group were not significant statistically. The Bax and activated caspase-3 expressions in endometrial gland in the model group became significantly lower than that of the control group （P〈0.01）, whereas those in the treatment group were significant higher than that of the model group （P〈0.01）. However, the Bax and activated caspase-3 expressions in endometrial gland were similar in both treatment and control groups. Conclusion： Promoting the increases in Bax and activated caspase-3 expressions in the endometrial gland and bringing into balance between apoptosis and proliferation of the glandular cells at the implantation window pha

关 键 词：blastocyst implantation dysfunction APOPTOSIS proliferating cell nuclear antigen （PCNA） Bax Bcl-2 Caspase-3 Bushenyiqihexue Formula （Formula for Tonifying the Kidney  Replenishing qi and Harmonizing Blood  FTKRQHB） 

分 类 号：R271.9[医药卫生—中西医结合]