不同时间去势对大鼠CIA发病情况的影响  

Effects of Castration at Different Time on Presentation of Collagen-induced Arthritis in Rat

作　　者：鞠大宏[1] 赵宏艳[1] 王燕[1] 肖诚[2] 查青林[3] 张继东[4] 董鑫鑫[3] 吕诚[5] 刘梅洁[1] 李艳[1] 刘红[1] 于峥[1] 潘贵超[6] 吕爱平[5] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]中日友好医院临床医学研究所,北京100029 [3]江西中医学院,江西南昌330004 [4]天津骨科医院,天津300211 [5]中国中医科学院中医临床基础医学研究所,北京100700 [6]北京市昌平区华一医院,北京102208

出　　处：《中国中医基础医学杂志》2010年第8期658-661,667,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家科技支撑计划重点课题(2006BAI08B01-01);"重大新药创制"科技重大专项(2009ZX09502-019)

摘　　要：目的:探讨肾虚痹证(类风湿性关节炎)病证结合动物模型的制作方法,为研究中医药治疗肾虚痹证(类风湿性关节炎)的作用及其机理提供实验工具。方法:选用6周龄SPF级SD大鼠100只,雌雄各半,除对照组大鼠外,分别切除大鼠的双侧睾丸或卵巢,制作肾虚动物模型。分别在去势1～4周后,采用尾根部皮下注射Ⅱ型胶原与不完全弗氏佐剂混合物的方法诱导出关节炎模型,从而对不同去势时间(即不同肾虚状态)下胶原诱导性关节炎(CIA)发病情况进行比较研究,并采用ELISA等方法对其机理进行初步探讨。结果:随着去势时间的延长,即肾虚程度加重,CIA有效发病率和发病程度亦随之增加;在去势4周后其有效发病率就可达到95%,且发病程度明显高于同期未去势大鼠。在有效发病率和发病程度上,雌雄之间无明显差异。结论:肾虚在CIA发病过程中起着重要作用,在制作肾虚痹证(类风湿性关节炎)病证结合动物模型时,大鼠去势4周时用CⅡ进行免疫诱导,即可制作出有效发病率高、发病程度重的肾虚痹证(类风湿性关节炎)病证结合大鼠模型。Objectives：Take RA（Rheumatoid arthritis）as experimental subject,to quest for the method of making the deficiency of kidney rat model combined with disease and syndrome.Methods：Choose SPF level 6 weeks SD rats 100,male and female cutting off testiculus and ovary respectively.Then use cattle Ⅱ type collagen to make the rat arthritis model after 1 week,2 weeks,3 weeks,4 weeks.Observing it＇s arthrosis accumulate points and microscope changes,compare the morbidity and disease severity in different terms of deficiency of the kidney.Results：With the time of castration going by,the effective morbidity and AI is increasing gradually.After 4 weeks of castration,the the effective morbidity can get 95%,and the AI in the castrated rats after 4 weeks is higher than in the non-castrated rat.The effective morbidity and AI is no difference between femal and male rat.Conclusions：kidney deficiency is an import factor in the deveploping of CIA,We can choose the 4 weeks emasculation rats for disease-syndrome conbination animal model of kidney deficiency Bi syndrom（rheumatoid arthritis）.At this time,we can make an model of kidney deficiency Bi syndrom（rheumatoid arthritis）with high effective morbidity and AI.

关 键 词：痹证 类风湿性关节炎 去势手术 胶原诱导性关节炎 病证结合动物模型 

分 类 号：R285.5[医药卫生—中药学]