超声萃取海螵蛸净化装置色谱分析农药残留技术研究  

Research on Chromatographic Analysis of Pesticide Residues with Technology of Ultrasonic Extraction Cuttlebone Purification Equipment

作　　者：孙峰[1] 张晓东[1] 周荣荣[1] 

机构地区：[1]日照市疾病预防控制中心,山东日照276826

出　　处：《预防医学论坛》2010年第9期820-822,861,共4页Preventive Medicine Tribune

摘　　要：[目的]利用海螵蛸理化性质，采用色谱固相萃取、净化的作用，通过超声振动对样品进行预处理，建立快速、准确、有效的方法，借助气相色谱分析食品中农药残留量。[方法]根据超声波空泡理论，利用海螵蛸特有吸附和解吸附的作用，提高有机相的萃取效率。[结果]方法线性：a=4803。b=12．62，r=0．9996，回收率：97．0％～990．4，RSD=5．02％，方法检出限9．86×10^-4μg／L。[结论]超声萃取海螵蛸净化装置的应用，解决仪器分析精度与样品预处理不统一的问题，并对样品中所含蛋白、色素和其他干扰物质，有选择的萃取、净化分离被分析组分。方法快捷、准确、可靠。[Objective]To establish a rapid, accurate, effective method, with the physical and chemical properties of cuttlebone,by use of solid-phase extraction, purification, and pre-treatment of the samples with ultrasonic vibration, and use of chromatography to analyze pesticide residues in food. [Methods]According to theory of ultrasonic cavitation, improving the extraction rate of organic phase with cuttlebone, s unique effect of adsorption and desorption, [Results]There was a good linear correlation： a = 4 803, b = 12.62, r = 0. 999 6, the recoveries ranged from 97.0% to 99 %, with relative standard deviations 5.02 %, the detection limit was 9.86 ×10^-4μg/L. [Conclusion]The application of ultrasonic extraction cuttlebone purification equipment in solving the problem of incompatibility between instrumental analysis precision and sample pre-treatment, and also selectively extracting and purifying and isolating samples which contain proteins, pigments, and other disruptors. This method is rapid, accurate and reliable.

关 键 词：超声萃取 海螵蛸 色谱 农药残留 

分 类 号：R139.3[医药卫生—劳动卫生]