束花石斛种子超低温保存的研究  

Study on Cryopreservation of Dendrobium chrysanthum (Orchidaceae) Seeds

作　　者：何明高[1,2] 王瑞霞[3] 宋希强[2,4] 宋松泉[3] 张如莲[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]海南大学园艺园林学院,海南儋州571737 [3]中国科学院植物研究所,北京100093 [4]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南儋州571737

出　　处：《云南植物研究》2010年第4期334-338,共5页Acta Botanica Yunnanica

基　　金：中国科学院重要方向性项目(KZCX2-YW-414);五唇兰种质资源收集与种子保存技术研究(YJS-2008-S030);国家科技支撑计划(2006BAD07B05)

摘　　要：以濒危兰科物种野生束花石斛(Dendrobium chrysanthum)的成熟种子为材料,研究了PVS2植物玻璃化液对其萌发的作用,以及快速冷冻法和玻璃化法对种子超低温保存的影响。结果表明,种子的萌发率随着PVS2处理时间的延长而下降。PVS2预处理能明显地增加种子的超低温耐性。当预处理时间为15～45min时,种子的超低温耐性随预处理时间的延长而增加;当预处理时间长于60min后,种子的超低温耐性随预处理时间的延长而下降。经液氮保存后,存活的种子能萌发成为正常的幼苗。结论是经PVS2预处理45min后,成熟的束花石斛种子能成功地进行超低温保存。This work aimed to study the role of plant vitrification solution 2 （PVS2） on seed germination,and the effect of rapid freezing and vitrification treatment on seed cryopreservation of the wild mature seeds of Dendrobium chrysanthum,an endangered orchid species. The results indicated that the seeds germination decreased with increasing treatment time of PVS2. Cryo-tolerance of seeds was obviously increased by PVS2 pre-treatment. When pre-treatment time was below 45 min,seeds cryo-tolerance increased with increasing pre-treatment time of PVS2,while it decreased after 60 min. The survival seeds could germinate and develop into normal seedling after storage in liquid nitrogen. In conclusion,mature D.chrysanthum seeds could be successfully cryo-preserved after 45 min pre-treatment with PVS2.

关 键 词：超低温保存 束花石斛 兰科植物种子 快速冷冻 玻璃化 

分 类 号：S682.31[农业科学—观赏园艺]