烟区轮作与连作土壤细菌群落多样性比较  

Diversity comparison of soil bacteria communities in rotation and continuous flue-cured tobacco cropping

作　　者：贾志红[1,2,3] 易建华 苏以荣[1] 曾军英[4] 

机构地区：[1]中国科学院亚热带农业生态研究所,湖南长沙410125 [2]中国科学院研究生院,北京100049 [3]湖南中烟工业有限责任公司,湖南长沙410007 [4]湖南怀化学院,湖南怀化418000

出　　处：《生态环境学报》2010年第7期1578-1585,共8页Ecology and Environmental Sciences

基　　金：中国科学院知识创新工程重要方向资助项目(KSCX2-YW-N-48-1);湖南中烟工业有限责任公司科研资助项目(2007-YC-001)

摘　　要：采用从土壤中直接提取土壤细菌总DNA,并用细菌16SrDNA特异性引物进行PCR扩增和变性梯度凝胶电泳（DGGE）的方法,对比研究了贵州福泉烟区轮作和连作对土壤细菌群落多样性的影响,每个处理样品3次重复在DGGE图谱中相似性较高,基本聚在一起,从整体证明了试验操作方法较为精确。由DGGE图谱可知,轮作土壤微生物条带数量15-31条不等,平均为23条。连作土壤微生物条带数量从7-25条不等,平均为18条。分别从每条泳道的条带数和光密度值两方面,对细菌群落多样性指标进行了比较。结果表明,轮作处理细菌群落丰富度指数均大于连作处理,轮作Shannon-wiener和辛普森指数（1/D）均大于连作,表明轮作微生物多样性较连作高,轮作方式可以提高植烟土壤细菌群落的多样性。Bacterial diversities in soils of rotation and continuous flue-cured tobacco cropping were measured by using 16SrDNA PCR-DGGE profiling.Crude bacterial DNA was extracted from soil.Amplification of 16S rDNA by PCR was made by a pair of specific bacterial primers,and then cDNA products were separated by denaturing gradient gel electrophoresis.The bands on gel in-dicated the bacterial diversity in the soils of rotation and continuous flue-cured tobacco cropping.The high similarities in three repli-cates suggested the reliability of method in the experiments.Based on the DGGE profile,15 to 31 bands（averagely 23）were ob-served in the soils of rotation cropping,while the corresponding value is from 7 to 25,avergely 18 in continuous cropping.According to the electrophoretic bands and optical density value,it was suggested that bacterial community of richness was higher in rotation than continuous tobacco cropping.The Shannon-wiener index and Simpson index（1/D）were also higher in rotation than continuous cropping.Taken together,our resutls indicated rotation tobacco cropping could improve the bacterial diversity of soils.

关 键 词：轮作 连作 PCR-DGGE 土壤细菌群落 多样性 烤烟 

分 类 号：S154.36[农业科学—土壤学]