槟榔碱对高糖诱导血管内皮依赖性舒张功能损伤的保护作用  

Arecoline Prevents the Injury of Endothelium Dependent Relaxation Induced by High Glucose

作　　者：王佑权[1] 周寿红[2] 

机构地区：[1]南华大学附属第二医院肿瘤外科,湖南省衡阳市421001 [2]南华大学医学院生理学教研室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2010年第6期381-384,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨槟榔碱对高糖诱导的内皮依赖性舒张功能损伤的保护作用及其可能的机制。方法采用离体血管环灌流方法 ,观察槟榔碱对高糖诱导的大鼠血管内皮依赖性舒张功能损伤的影响以及血管组织中一氧化氮和丙二醛含量以及超氧化物歧化酶活性的影响。同时,观察M受体阻断剂阿托品和一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯对槟榔碱作用的影响。结果高糖(44mmol/L)处理组显著降低了乙酰胆碱诱导的大鼠血管内皮依赖性舒张反应,槟榔碱(0.001、0.01和0.1mmol/L)以浓度依赖的方式抑制了高糖诱导的血管内皮依赖性舒张反应损伤。而阿托品和N-硝基-L-精氨酸甲酯取消了槟榔碱的作用。高糖(44mmol/L)处理组显著降低了血管组织中一氧化氮含量和超氧化物歧化酶活性而增加了血管组织中丙二醛含量,槟榔碱(0.1mmol/L)逆转了高糖的这种作用。结论槟榔碱抑制了高糖诱导的血管内皮依赖性舒张功能损伤,其机制可能与槟榔碱能激动M受体,增加一氧化氮的释放,抑制氧化应激有关。Aim To investigate the effect of arecoline on the injury of endothelium dependent relaxation induced by high glucose of isolated thoracic aorta of SD rats and explore the potential mechanism. Methods Organ baths in thoracic aortic rings of SD rats were used to investigate the effect of different concentration arecoline on the injury of endo- thelium dependent relaxation induced by high glucose. The levels of nitric oxide （ NO ） and malonaldehyde （ MDA ） and the activity of superoxide dismutase （SOD） in the isolated thoracic aorta tings were measured. The M receptor inhibitor atropine and nitricoxide synthase inhibitor N-nitrio-L-argine methyl ester （L-NAME ） were used in the study. Results Compared with the control group , endothelium dependent relaxation induced by aeetylcholine of isolated thoracic aorta of SD rats was significantly decreased in high glucose group. The injury of endothefium dependent relaxation induced by high glucose was inhibited by arecoline in concentration-dependent manner. But the effect of arecoline was abolished by atro- pine and L-NAME. The levels of NO and the activity of SOD were decreased and the levels of MDA was increased in the isolated thoracic aorta rings by high glucose, but the effect of high glucose was reversed by arecoline. Conclusion Arecoline prevents the injury of endothelium dependent relaxation induced by high glucose, which mechanisms may be re- lated to activation of M receptor, increase of NO and inhibition of oxidative stress induced by arecoline.

关 键 词：槟榔碱 高糖 内皮依赖性舒张反应 一氧化氮 丙二醛 超氧化物歧化酶 

分 类 号：R96[医药卫生—药理学]