新疆居民HBV基因型的分布及其与临床表现关系的初步研究  

Preliminary study on distribution and relationship with phenotype of hepatitis B virus genotype detected from Xinjiang uygur autonomous region in China

作　　者：李蕾[1] 费永亮[2] 伊瑶[2] 陈斯勇[2] 毕胜利[2] 

机构地区：[1]新疆维吾尔自治区人民医院检验科,乌鲁木齐830001 [2]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2010年第4期257-259,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 了解新疆地区乙肝患者HBV基因型的分布及其与临床表现的关系.方法 从2000余份血清中筛选出HBsAg阳性血清200份.利用巢式PCR法扩增血清中HBV DNA S基因进行测序并分型;统计分析基因型分布及与临床表现的关系.结果 新疆HBV有B、C、D三个基因型,其中B基因型10例（7.8%）,C基因型58例（45.7%）,D基因型59例（46.5%）.C基因型中汉族51例（87.9%）,D型中汉族27例（45.7%）,维吾尔族24例（40.7%）.结论 新疆地区HBV基因型以C、D型为主,少量为B型.C型以汉族为主,D型中汉族和维吾尔族占多数.C型与更严重的疾病及转归有关.Objective To determine the main genotype of hepatitis B virus （HBV） in Xinjiang.Methods 200 HBsAg positive serum specimens were detected from more than 2000 serum of Xinjiang inhabitants, and HBV S gene was detected by using nPCR amplifying, and compared with the standard S region HBV nucleotide sequences of genotypes A-H retrieved from GenBank, then analyzed by MEGA3 and SAS3 software. Result Gene in 127 （63.5%） serum specimens was detected from 200 samples. Among 127 serum specimens, 10 （7.8%） was genotype B, 58 （45.7%） was genotype C, and 59 （46.5%） was genotype D. Han People were 51 （87.9%） in genotype D and 27（45.7%） in genotype D, Uygur were 24 in genotype D. Conclusion Genotype B（40.7%）, C and D have been found in Xinjiang. Genotype C was more prone to causing severe liver disease.

关 键 词：肝炎病毒 乙型 基因型 人种群 临床医学 

分 类 号：R512.62[医药卫生—内科学]