银杏内酯B对星形胶质细胞释放NO、IL-6及趋化因子RANTES的影响  

Effect of ginkgolide B on the production of NO, IL-6 and RANTES from astrocytes

作　　者：彭珊瑛[1] 廖文辉[1] 聂珍贵[2] 刘洋[1] 王霖[1] 王凤[1] 王文杰[1] 

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,北京100050 [2]首都医科大学燕京医学院,北京101300

出　　处：《药学学报》2010年第9期1103-1108,共6页Acta Pharmaceutica Sinica

基　　金：国家高技术研究发展计划(863计划)资助项目(2002AA233081)

摘　　要：考察银杏内酯B对星形胶质细胞释放NO、IL-6及趋化因子RANTES的影响,探讨在炎性刺激条件下,银杏内酯B对星形胶质细胞的保护作用机制。采用Griess试剂检测培养上清液中NO的含量,ELISA法测定IL-6及RANTES的含量,RT-PCR法测定IL-6及RANTESmRNA的表达。结果显示,LPS可显著刺激大鼠原代星形胶质细胞释放NO,银杏内酯B可显著抑制LPS刺激的NO释放;IL-1β可显著刺激U251星形胶质瘤细胞IL-6和RANTES的生成,并增加其mRNA的表达,银杏内酯B可明显抑制IL-1β诱导的IL-6和RANTES的生成,并可显著抑制IL-1β和RANTESmRNA的表达。因此,银杏内酯B可能是通过减少NO的生成,降低星形胶质细胞炎症因子IL-6mRNA和细胞趋化因子RANTESmRNA的表达,从而减少IL-6及RANTES的产生,减轻炎症反应的程度。结果表明,银杏内酯B可能通过降低AD发病和进展过程中的与星形胶质细胞相关的慢性炎症反应,从而缓解AD症状。This study is to explore the effect of ginkgolide B （BN52021） on the production of nitric oxide （NO）, interleukin （IL）-6 and regulated upon activation normal T cell expressed and secreted （RANTES） from astrocytes induced by stimulators. Primary cultured rat astrocytes were stimulated with lipopolysaccharides （LPS）, the production of NO was assayed using Griess reaction; U251 cells were stimulated with IL-1β, the contents of IL-6 and RANTES in the supernatant were measured using ELISA. The mRNA expressions of IL-6 and RANTES were detected using RT-PCR. LPS （10 ng·mL？1 to 10 μg·mL？1） could stimulate rat astrocytes to produce NO in a dose-dependent manner. Ginkgolide B at the concentrations of 0.1？10 μmol·L？1 were shown to decrease NO production significantly. IL-1β could induce the mRNA expression and protein secretion of IL-6 from U251 cells, as well as RANTES. Ginkgolide B at concentrations of 0.1？10 μmol·L？1 were shown to inhibit RANTES secretion, and to inhibit mRNA expression of IL-6 and RANTES at concentration of 10 μmol·L？1. Ginkgolide B has inhibitory effect on the production of NO, IL-6 and RANTES from astrocytes treated with inflammatory stimulators.

关 键 词：银杏内酯B NO IL-6 RANTES 星形胶质细胞 

分 类 号：R963[医药卫生—微生物与生化药学]