玻璃化法和程序慢冻法冻融人卵裂期胚胎的妊娠结局及新生儿的比较  

Comparison of Glass Micropipette Vitrification with Slow-cooling Method used in Human Day-3 Cleavage-stage Embryos:Pregnancy and Neonatal Outcomes

作　　者：冯贵雪[1] 张波[1] 舒金辉[1] 周红[1] 周莉[1] 甘贤优[1] 刘茵[1] 

机构地区：[1]广西壮族自治区妇幼保健院生殖中心,广西南宁530003

出　　处：《实用妇产科杂志》2010年第9期688-691,共4页Journal of Practical Obstetrics and Gynecology

基　　金：广西自然科学基金(编号:桂科攻0897007;桂科自0542058;桂科自0832183);广西卫生厅课题(编号:重200947Z2007013)

摘　　要：目的:比较以玻璃微细管为载体的玻璃化法和程序慢冻法冷冻保存人卵裂期胚胎的临床效果、妊娠结局及新生儿情况。方法:将接受体外受精-胚胎移植患者的剩余卵裂期优质胚胎进行玻璃化法或程序慢冻法冷冻,比较复苏后的胚胎存活率、种植率、妊娠率、流产率、活胎分娩率、出生缺陷率、新生儿平均出生体重及孕周。结果:玻璃化法移植的胚胎数目显著少于程序慢冻法(2.32±0.66个vs 2.51±0.60个,P=0.029),但胚胎种植率、临床妊娠率明显高于程序慢冻法(19.03%vs 10.74%,P=0.0005;34.40%vs 23.21%,P=0.0193)。在妊娠周期中,两种冷冻方法的流产率、活胎分娩率、新生儿出生体重、孕周及新生儿出生缺陷率比较,差异均无统计学意义(P>0.05)。结论:以玻璃微细管为载体的玻璃化法能有效冷冻保存人卵裂期胚胎,其冷冻效率优于程序慢冻法,并且未见新生儿先天畸形。Objective. To compare and evaluate the clinical efficiency and neonatal outcomes after glass micropipette vitrification or slow-cooling cleavage stage embryo. Methods：Cleavage stage good quality embryos were cryopreserved for embryo transfer in future by glass micropipette vitrification or slow-cooling method. Post-thaw embryo survival, implantation and pregnancy rates, abortion rate, live birth rate, birth defect rate,mean neonatal birth weight and mean gestation age were compared. Results：The number of transfered embryo by vitrification was significantly less than that by slow-cooling method （2.32 ±0.66 vs 2.51 ± 0.60, P = 0. 029）, but the implantation rate and pregnancy rate by vitrification were significantly higher than those by slow-cooling method （ 19.03% vs 10.74%, P = 0. 0005 ; 34.40% vs 23.21%, P = 0. 0193 ）. The miscarriage rate, live birth rate , birth defect rate , mean neonatal weight and gestational age by vitrification were no difference compared with those by slow-cooling method （ P 〉 0.05）. Conclusions：Glass micropipette vitrification is an more efficient method for cryopreservation of human cleavage stage embryos than slow-cooling method, and no increased risk of birth defects is found.

关 键 词：胚胎 玻璃化冷冻 玻璃微细管 妊娠结局 新生儿 

分 类 号：R71[医药卫生—妇产科学]