丝素蛋白的相关生物学性能研究  

Study on Biological Performance of Silk Protein

作　　者：徐广涛[1] 林天送[2] 周新妹[1] 于海宁[2] 徐国文 吴晓薇[1] 郑漾[1] 俞杭斌[1] 陈士票[1] 

机构地区：[1]嘉兴学院医学院病理学与病理生理学系,314001 [2]浙江大学药学院药学系 [3]浙江湖州新天丝生物技术有限公司

出　　处：《天津医药》2010年第9期790-793,共4页Tianjin Medical Journal

基　　金：浙江省自然科学基金重大专项(项目编号:2008C1302-2)

摘　　要：目的:检测丝素蛋白的各项生物学性能,为开发丝素蛋白的医药价值提供依据。方法:通过人工方法将丝素蛋白制成丝素肽、350nm丝素粉、3μm丝素粉、大分子质量丝素肽和纳米丝素粉(<100nm)5种不同分子质量、不同大小的颗粒,配制相应的溶液。利用平板菌落计数法、吸光度法、饱和碳酸钙溶液吸湿率法、致敏接触及激发接触法分别检测丝素蛋白的抑菌活性、抗紫外线能力、吸湿性、保湿性及皮肤致敏性。结果:5种丝素蛋白溶液均有一定抑菌性,除350nm丝素粉外,其余丝素蛋白样品溶液对大肠杆菌的生长抑制效果优于对金黄色葡萄球菌的生长抑制效果。经紫外线照射1h后,丝素肽、3μm丝素粉、大分子质量丝素肽的紫外线吸收仍较强。大分子质量丝素肽和丝素肽的吸湿能力较好,48h吸湿率分别为44.78%和35.30%。3μm丝素粉和丝素肽的保湿能力更强,48h保湿率分别为82.29%和78.91%。丝素蛋白液对豚鼠皮肤均无致敏性。结论:丝素蛋白的生物学性能可望在医药领域得到更深、更广地开发和利用。Objective： To detect bacteriostatic activity, uvioresistant ability, hygroscopicity, moisture and dermal sensitization of silk protein,and the scientific basis for medical data thereof. Methods： By artificial means,silk protein was made of 5 different molecular weights, different sizes particles including silk fibroin peptide, 350 nm fibroin powder, 3 μm silk powder, silk peptide molecules and nanowires powder （100 nm）. The corresponding solutions were prepared. The related biological activity was detected by using plate bacterial colony count, absorption, moisture absorption rate of saturated calcium carbonate solution, allergen exposure and stimulate contact assay. Results： There were certain antibacterial abilities in 5 kinds of silk proteins. The inhibitory effect was excellent on E. coli than Staphylococcus aureus. There were strong abilities to absorb ultraviolet radiation in silk peptide, 3 μm silk protein powder and high molecular weight silk peptide after 1 h ultraviolet irradiation. High molecular weight silk peptide and silk peptide showed a better hygroscopic, and the hygroscopic rates were 44.78% and 35.30% in 48 h. The 3 μm silk protein powder and silk peptide showed the better moisture retention, and the moisture rates were 82.29% and 78.91% in 48 h. Silk protein was no skin sensitization in guinea-pig skin. Conclusion： The biological properties of silk protein are expected to be deeper, wider development and application in the medical field.

关 键 词：丝素蛋白 微生物敏感性试验 紫外线 可湿性 大肠杆菌 豚鼠 

分 类 号：R318.08[医药卫生—生物医学工程]