地塞米松对小鼠DC2.4细胞表型及功能的影响  

Effects of dexamethasone on the phenotype and function of mouse DC2.4 cell line

作　　者：宫玉波[1] 黄一飞[1] 黎燕[2] 韩根成[2] 李育蓉[2] 王大江[1] 杜改萍[1] 余继锋[1] 宋静[1] 

机构地区：[1]解放军总医院眼科,北京100853 [2]军事医学科学院基础医学研究所分子免疫室,北京100850

出　　处：《免疫学杂志》2010年第9期759-763,共5页Immunological Journal

基　　金：国家自然科学基金项目(30973245)

摘　　要：目的研究地塞米松(dexamethasone,Dex)对DC2.4细胞表型及功能的影响。方法取生长状态良好的DC2.4细胞,随机分为4组,A组为对照组;B、C、D组分别为50、100、200μg/L地塞米松组,每组DC2.4细胞培养6d。用流式细胞术测定DC表面CD80,CD86,Galectin-9及PD-L1的表达,ELISA方法检测其培养上清IL-12的浓度,用混合淋巴细胞培养法检测其对同种异体T细胞的刺激能力。结果与对照组相比,经不同剂量地塞米松修饰的DC2.4细胞其表面CD80,CD86,Galectin-9及PD-L1的表达无明显改变,但地塞米松用药组显著减少IL-12的分泌及抑制刺激同种异体T细胞增殖的能力。结论 DC2.4细胞是一细胞表型稳定的细胞系;地塞米松没有明显改变其细胞表型,但抑制其细胞因子的分泌,降低其刺激同种异体反应T细胞增殖的能力。We aim to study the effect of dexamethasone （Dex） on phenotype and function of mouse DC2.4 cell line. DC2.4 cells which grew well were divided into 4 groups：control,50 μg/L,100 μg/L,and 200 μg/L Dex groups. And DC2.4 cells in every group were cultured for 6 days. Flow cytometry indicated there was no significant difference of CD80,CD86,Galectin-9,and PD-L1 expressions between control group and DC2.4 cells treated with different doses of Dex. However,ELISA and mixed lymphocyte reaction showed that Dex treatment reduced IL-12 level secreted by DC and inhibited the stimulating activity of DC2.4 cells on allogeneic T cells significantly. We concluded that the DC2.4 is a phenotype stable cell line; Dex does not significantly change the cell phenotype,but inhibits the secretion of cytokines,reduces the stimulation of allogeneic T cell proliferation response capacity.

关 键 词：DC2.4 半乳糖凝集素-9 程序化死亡因子配体1 地塞米松 免疫耐受 

分 类 号：R965[医药卫生—药理学]