人肝再生增强因子对IL-10分泌的影响  

Effects of human augmenter of liver regeneration on IL-10 secretion

作　　者：王新国[1,2,3] 袁建国[4] 刘杞[1,2] 

机构地区：[1]重庆医科大学附属二院 [2]重庆医科大学病毒性肝炎研究所,教育部感染性疾病分子生物学重点实验室,400010 [3]杭州市第六人民医院,310014 [4]山东省聊城市人民医院传染科,252000

出　　处：《免疫学杂志》2010年第9期770-771,775,共3页Immunological Journal

基　　金：国家自然科学基金(30570826);重庆市自然科学基金(2008BB5228)

摘　　要：目的观察人肝再生增强因子对IL-10分泌的影响。方法用梯度离心法分离正常人PBMC,分成正常组、hALR组、ConA刺激组和ConA+hALR组。培养16h后,用MTT法观察细胞增殖情况,双抗夹心酶联法检测上清中细胞因子IL-2,IL-10水平。结果 ALR显著抑制ConA对细胞的增殖作用的同时,明显促进细胞因子IL-10分泌和显著抑制细胞因子IL-2分泌。结论 ALR可能通过分泌IL-10和抑制IL-2分泌而发挥免疫抑制作用。To observe the effect of human augmenter of liver regeneration （hALR） on IL-10 secretion,PBMCs were isolated from healthy volunteers by gradient centrifugation and divided into normal,ALR,ConA and ALR＋ConA groups. The proliferation of PBMCs and the levels of IL-2 and IL-10 in the supernatant were detected by MTT and Double-antibody Sandwich Enzyme-linked Immunosorbent Assay respectively when cells were cultured for 16 hours. Correlation between IL-2 and IL-10 was analyzed. As a result,ALR promoted the PBMCs to secret cytokine IL-10,while inhibited the secretion of the cytokines IL-2 and significantly inhibited the ConA positive effect on cell proliferation. In a conclusion,hALR plays immunosuppressive effects possibly through promoting IL-10 secret and inhibiting IL-2 secretion.

关 键 词：人肝再生增强因子 IL-2 IL-10 PBMC 

分 类 号：R392.7[医药卫生—免疫学]