利用碱性成纤维细胞生长因子包裹的磁性纳米粒对大鼠急性骨骼肌钝挫伤后肌肉收缩力与松弛特性恢复的影响研究  

Study of the Contractive Strength and Muscle Relaxation in Rat Skeletal Muscles after Acute Contusion by Using Basic Fibroblast Growth Factor-Coated Magnetic Nanoparticles

作　　者：葛新发[1] 潘卫东[2] 董贵俊[1] 

机构地区：[1]山东体育学院研究生部,济南250063 [2]中国科学院电工研究所生物医学工程部

出　　处：《中国运动医学杂志》2010年第5期538-541,546,共5页Chinese Journal of Sports Medicine

基　　金：山东省自然科学基金(Y2007C116)

摘　　要：目的:研究碱性成纤维细胞生长因子(bFGF)包裹的磁性纳米粒对大鼠急性骨骼肌钝挫伤后肌肉收缩力与松弛特性恢复的影响。方法:通过免疫化学偶联技术,制备重组bFGF包裹的磁性纳米粒。将雄性Wistar大鼠随机分为自然愈合组、生理盐水组、小牛血清白蛋白(BSA)包裹磁性纳米粒组、bFGF包裹磁性纳米粒组和bFGF直接注射组,采用重物下落装置建立大鼠急性骨骼肌钝挫伤模型,按上述分组方式分别于损伤部位皮下注射药物或不作处理,在外加磁场的牵引下靶向聚焦至损伤部位。在损伤后第2、10、17、24和30天分别取样,测定和计算肌肉收缩力和应力松弛。结果:磁纳米化的bFGF对大鼠腓肠肌钝挫伤后第17、24天的收缩力有明显提高作用;在损伤后第2天,磁纳米化的bFGF即能使应力松弛恢复接近正常肌肉水平;与bFGF直接注射组相比,bFGF包裹磁性纳米粒对损伤肌肉收缩力的提高更明显(P<0.05,第17天)。结论:磁纳米化的bFGF比直接注射bFGF能明显改善大鼠急性骨骼肌钝挫伤后肌肉收缩应力和恢复应力衰减。Objective To study the effects of basic fibroblast growth factor（bFGF）-coated magnetic nanoparticles on the recovery in rat skeletal muscles after acute contusion. Methods Recombinant bFGF-coated magnetic nanoparticles were produced by immunochemical coupling techniques. Male Wistar rats were randomly divided into natural healing group,physiological saline group,BSA-coated magnetic nanoparticles group,bFGF-coated magnetic nanoparticles group and direct injection bFGF group. The acute skeletal muscle contusion was produced by heavy objects dropping devices. Subcutaneous injection or blank control were performed respectively in different groups,targeted and focused at the injured site with the guidance of external magnetic fields. The muscles were sampled at day 2,10,17,24 and 30 respectively after contusion,and then the muscle contractive strength and relaxation were measured and calculated. Results Magnetically nanocrystallized bFGF significantly increased the muscle contractive strength at day 17 and day 24 in gastrocnemius after contusion. From day 2 after contusion,magnetically nanocrystallized bFGF restored the muscle relaxation back close to the normal level. Compared with the direct injection bFGF group,BFGF-coated group significantly increased the muscle contractive strength at day 17 （P 0.05）. Conclusions Magnetically nanocrys tallized bFGF could significantly enhance the contractive strength,restore the muscle relaxation and effectively improve the healing process of injured muscles caused by acute skeletal muscle contusion.

关 键 词：碱性成纤维细胞生长因子 磁性纳米粒 骨骼肌钝挫伤 

分 类 号：R873[医药卫生—运动医学]