大鼠Delta1基因转染对树突状细胞生物学特性的影响  

Effects of delta1 gene transfection on biological behaviors of rat dendritic cells

作　　者：郑凯[1] 谭建明[1] 吴卫真[1] 杨顺良[1] 

机构地区：[1]南京军区福州总医院全军器官移植中心,福建福州350025

出　　处：《细胞与分子免疫学杂志》2010年第9期846-848,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省自然科学基金资助项目(C0510033)

摘　　要：目的:探讨大鼠delta1基因在树突状细胞(DCs)中的表达及其意义。方法:利用脂质体基因转染技术,将含大鼠delta1基因片段的真核表达载体pcDNA3.1(+)/delta1转染入DCs中,采用流式细胞术(FCM)和混合淋巴细胞培养(MLR)等方法检测delta1基因转染对DCs生物学特性的影响。结果:比较实验组、空载体组和对照组DCs在超微结构、细胞周期和细胞生长速度上无明显差异。FCM检测显示,delta1并不影响DCs表面共刺激分子CD80、CD86和MHC-II类分子Iad的表达;但delta1基因转染的DCs分泌IL-12的水平明显降低(P<0.05),即使加入LPS刺激后,IL-12的分泌也无明显增加。结论:Delta1/Notch信号表达的增加,不影响DCs的分化与成熟,对其免疫学功能起负性调节作用。Delta1的可能机制为其通过抑制DCs分泌IL-12,来促使Th1/Th2细胞亚群的分化倾向于Th2细胞方向。AIM： To explore the effects of Delta1 gene on rat bone marrow-derived dendritic cells （DCs）.METHODS： pcDNA3.1/delta1 plasmid was transfected into Rat DCs with lipofectamine gene transfection method.The expression of activation markers（Iad,CD80 and CD86） were examined by flow cytometry,the release of cytokines IL-12 were examined by ELISA and the stimulatory capacity of the resulting DCs in vitro were examined by mixed lymphocyte reaction（MLR）.RESULTS： The biological character of DCs in experimental group,empty vector group and control group were examined by electron microscope,flow cytometry and cell growth rate counting.There were no differences of cell cycles,ultrastructure and cell growth rate among these three group cells（P0.05）.It stated that delta1 gene had no effect on the growth of DCs.Even at the stimulation of LPS,In the delta1 transfected DCs,we observed no significant effects on the expression of activation markers（Iad,CD80 or CD86）.The release of IL-12 in DCs/delta1 was reduced compared with control DCs（P0.05）.The levels of IFN-γ and IL-2 of the DCs/delta1 were significantly lower than those of the DCs（P0.05）,and the levels of IL-4 and IL-10 were significantly higher than that in control DCs（P0.05）.CONCLUSION： Our data demonstrates that the delta1 gene transfection does not interfere with the differentiation or maturation of DCs,but reduce the ability to stimulating T cells,and the mechanism may be related to the decrease of Th1 /Th2 ratio by impairing the production of DCs-derived IL-12.

关 键 词：NOTCH信号 树突状细胞 免疫耐受 器官移植 

分 类 号：R392.12[医药卫生—免疫学]