^(60)Co-γ辐射对番茄离体子叶再生及不定芽分化的影响  

Effects of ^(60)Co-γ irradiation on in vitro cotyledon regeneration and adventitious bud differentiation of tomato

作　　者：朱宗文[1] 查丁石[1] 郭世荣[2] 朱为民[1] 叶文娣[3] 殷伯贤[3] 黄俭[3] 陈红辉[3] 

机构地区：[1]上海市农业科学院园艺研究所上海市设施园艺技术重点实验室,上海201106 [2]南京农业大学园艺学院,南京210095 [3]上海市金山区蔬菜技术推广中心,上海201500

出　　处：《上海农业学报》2010年第3期1-4,共4页Acta Agriculturae Shanghai

基　　金："十一五"国家科技支撑计划(2008BAD97B06)资助

摘　　要：用0～100 Gy的^(60)Co-γ射线对番茄品种‘1479'的组培无菌苗子叶进行辐射,研究^(60)Co-γ辐射对番茄组织培养中外植体分化的辐射效应。结果表明:^(60)★Co-γ射线辐射‘1479'子叶后,其出愈率、胚性愈伤诱导率和胚性愈伤的分化情况受到显著的影响。高辐射剂量(>50 Gy)显著抑制了子叶外植体的出愈率和胚性愈伤组织的形成,并对胚性愈伤中芽的分化产生了显著的影响,但对胚性愈伤不定根的诱导影响较小。番茄子叶外植体半致死剂量为50 Gy左右。Cotyledons of germ-free tissue-cultured plantlets of the tomato cultivar ＇ 1479＇ were irradiated by ^60Co-γ rays at different doses（0-100 Gy） in order to study the effects of ^60Co-γ irradiation on the differentiation of tomato explants during tissue culture. The results showed that ^60Co-γ irradiation had significant effects on the cotyledons in callus formation, and inductivity and differentiation of embryonic calli. The irradiation at a dose of more than 50 Gy significantly inhibited the callus inductivity of the cotyledons and the embryonic callus formation and had a significant impact on the shoot regeneration, but less influenced the root regeneration. It was concluded from the test data that the median lethal dose（LDs,,） to tomato cotyledons should be around 50 Gy.

关 键 词：~60Co-γ辐射 番茄 子叶 愈伤组织 分化 

分 类 号：S641.2[农业科学—蔬菜学] S603.6[农业科学—园艺学]